Patients who underwent laparoscopic partial nephrectomy in the First Affiliated Hospital of Nanjing Medical University from July 2016 to December 2017 were randomly divided into the enhanced recovery after surgery (ERAS) and the control group. The clinical indicators, duration of hospitalization, hospitalization cost, preoperative and postoperative anxiety, depression, and postoperative quality of life were compared between the two groups. The recovery time, hospitalization cost, incidence of complications and postoperative anxiety of patients in the ERAS group was lower than that of the control group. The satisfaction during hospitalization, scores of physical function, role function, emotional function and general health status of the ERAS group was also significantly increased. Applying the ERAS to patients undergoing laparoscopic partial nephrectomy can improve their prognosis, experience of medical treatment and life quality after surgery as well as have certain economic advantages.