Refinement and scientization is the future direction of the modern hospital operation and management. This study aims to establish a professional hospital operation management team, clarify the job content of the specialized operation assistant position, analyze the operating conditions of the hospital departments, evaluate the distribution of various resource, and participate in the management of department performance, process and project management. Medical service capabilities of the hospital departments increased and the cost were controlled appropriately; the staff enthusiasm enhanced after optimization of the performance scheme; the hospital departments process is optimized and the operation efficiency is significantly improved. The specialized operation assistants would find out problems in the operation of the hospital departments and improve it in time by evaluating human resources, financial resources, materials, space and process of departments. The establishment of the specialized operation assistant system promotes the transformation of the hospital management mode from experience?based and extensive to scientific and refined, and realizes the refined operation management.