Abstract:Objective: To investigate the expression and role of long non-coding RNA LINC01197 in gastric cancer. Methods: The expression of LINC01197 was analyzed and detected by datasets and gastric cancer tissues and paracancerous tissues. Human gastric epithelial cell line (GES-1)and different gastric cancer cell lines (SGC-7901, BGC-823, MGC-803 and AGS)were cultured respectively and the expression of LINC01197 was assessed . Cell lines SGC-7901 and BGC-823 were transfected with short-hairpin RNA targeting LINC01197 (shLINC01197),qRT-PCR assays were conducted to assess LINC01197 expression. CCK8 assays and colony formation assays were carried out to evaluate proliferation,transwell assays was performed to evaluate the capability of invasion and migration. Nude murine with tumor subcutaneously implanted was to evaluate cell growth of LINC01197 in vivo. mRNA-seq assays were used to find the differential genes after LINC01197 knockdown. The expression of SERPINA1 was detected by qRT-PCR and western blotting assays. Results:The expression of LINC01197 in gastric cancer tissues and cells was higher than that in paracancerous tissues and GES-1 cells respectively (P < 0.05). The cell proliferation, invasion and migration were significantly suppressed after LINC01197 knockdown in vitro (P < 0.05). The growth ability was significantly inhibited after LIN01197 knockdown in vivo (P < 0.01). The loss of LINC01197 substantially decreased the expression of SERPINA (P < 0.05). Conclusion:LINC01197 was up-regulated in gastric cancer tissues compared to their paired paracancerous tissues and in gastric cancer cell lines compared to GES-1. Downregulation of lncRNA LINC01197 would inhibit cell proliferation,invasion and migration capability in gastric cancer cells through partly inhibiting the expression of SERPINA1. LINC01196 might be a promising target for therapy in gastric cancer.