[Abstract] Objective: To investigate the distribution characteristics of HLA-A alleles in Han population from Chinese Marrow Donor Program (CMDP) Jiangsu Branch in 8 prefecture-level cities of Jiangsu Province. Methods: 27143 volunteers from CMDP Jiangsu Branch were genotyped at HLA-A locus using Polymerase Chain Reaction–Sequence Based Typing (PCR-SBT) method, frequencies of HLA-A alleles were separately calculated in 8 prefecture-level cities and compared. Results: A total of 86 HLA-A alleles were detected in Jiangsu Han population, among them 57,56,38,37,41,45,42,41 HLA-A alleles were separately detected in Changzhou, Nanjing, Zhenjiang, Yangzhou, Huai’an, Suqian, Xuzhou, Yancheng Han population. There was no significant difference in allele frequencies of A*02:01:01G, 11:01, 24:02, 30:01 and 33:03 among Nanjing, Zhenjiang and Yangzhou after statistical analysis. The common alleles in Nanjing and Zhenjiang were very close, which were A*11:01、24:02、02:01:01G、33:03、30:01、02:07、02:06、31:01、01:01. The common alleles in Changzhou were very close to Yangzhou, which were A* 11:01、24:02、02:01:01G、33:03、02:07、30:01、02:06、31:01、01:01.The common alleles in Yancheng and Suqian were very close, which were A* 11:01、24:02、02:01:01G、30:01、33:03、02:07、02:06、01:01, only difference in the order of A*02:01:01G and A*30:01. The common alleles in Xuzhou were very close to Huai’an, which were A*24:02、11:01、30:01、02:01:01G、33:03,only difference in the order of A*02:01:01G and A*30:01. Conclusion: The results reflected the distribution of HLA-A alleles of Jiangsu Han population from 8 prefecture-level cities and the alleles frequency distribution was basically consistent with the regional distribution. HLA-A alleles distribution of Xuzhou and Huai'an were similar to the Southern Area of Shandong, those of Nanjing, Zhenjiang, Changzhou and Yangzhou were similar to Shanghai and Zhejiang. These findings could serve as foundation for HLA-A related disease research, population anthropology in Jiangsu Han population.