Objective: To investigate whether conditioned medium from human amnion?derived mesenchymal stem cells (hAMSCs-CM) promotes human umbilical vein endothelial cells (HUVECs) angiogenesis via circ RNA-0001649 (circ-0001649) and its possible mechanism. Methods: HUVECs were devidede into control group, conditioned medium stimulation group(CM- group), conditioned medium stimulation after knockdown of circ-0001649 group(si-circ-0001649+ CM group) and knockdown control with conditioned medium stimulation group(si-NC+CM group), and the angiogenic and migratory abilities of HUVECs in each group were examined, as well as VEGFA and MMP9 protein levels. The mechanism of hAMSCs-CM promoting angiogenesis in HUVECs was also verified by knockdown of circ-0001649 followed by transfection of miR-203a-inhibitor for rescue experiments Results: hAMSCs-CM significantly increased the angiogenic and migratory capacity of HUVECs, the protein levels of VEGFA and MMP9, and the expression of intracellular circ-0001649. Knockdown of circ-0001649 reduced the effect of hAMSCs-CM on the angiogenesis and migratory of HUVECs. Bioinformatic analysis and rescue experiments revealed that circ-0001649 could promote the angiogenic and migratory ability of hAMSCs-CM on HUVECs by binding miR-203a and reducing the inhibitory effect of miR-203a on VEGFA and MMP9. Conclusion: HAMSCs-CM upregulated circ-0001649 expression in HUVECs cells, which function as a ceRNA via competitive binding to miR-203a, thus exerting a pro-angiogenic effect. Our findings provide a new way to investigate the mechanism of hAMSCs in promoting angiogenesis.