[Abstract] Objective To use bioinformatics methods to screen the differentially long non-coding RNA (LncRNA) in vaginal epithelial tissues of women with vaginal lubrication disorder (LD), and to construct patients with vaginal lubrication disorder based on the hypothesis of lncRNA-mRNA coexpression network to further explore the potential pathogenesis of patients with vaginal lubrication disorders, in order to provide new ideas for the diagnosis and treatment of patients with vaginal lubrication disorders. Methods Using ?log2FC?≥2 and Correct pValue＜0.05 to identify the expression profile of long-chain non-coding RNA and mRNA between vaginal lubrication disorders and normal control group, using Cytoscape software to construct a lncRNA-mRNA network of candidate lncRNAs, using Gene Ontology (GO) And KEGG Pathway to analyze the biological functions of mRNAs in coexpression network. Results A total of 499 up-regulated lncRNAs, 337 down-regulated lncRNAs, and 1582 up-regulated mRNAs, 633 were selected in the LD group and the normal control group based on the next-generation sequencing technology according to the criteria of ?log2FC?≥2 and Correct pValue＜0.05. Down-regulate the expressed mRNA. Subsequently, we constructed a lncRNA-mRNA coexpression network based on differentially expressed lncRNA and mRNA through 100 lncRNA and 311 mRNA. Finally, the functional enrichment analysis of the lncRNA-mRNA network shows that mRNA is mainly related to myocardial-related diseases and functions. Conclusion The pathogenesis of LD may be related to blood circulation dysfunction, local muscle dysfunction, and cGMP pathway, etc. This requires more studies at the cellular level, animal level and even human level to further confirm.