Abstract:Objective: To explore the role and potential molecular mechanism of microRNA (miR)-190 in aging-related energy metabolism disorders. Methods: Real-time fluorescent quantitative PCR (RT-PCR) was used to detect the expression level of miR-190 in the adipose tissue of aging mice; a cellular senescence model was constructed and RT-PCR was used to detect the changes of miR-190 in adipocytes; RT-PCR was used to detect the changes in thermogenesis genes in the adipocytes transfected with miR-190; the target genes of miR-190 were verified by the dual luciferase reporter gene experiment; the metabolic homeostasis was determined in aged mice injected with miR-190 antagomir. Results: During the aging process, the expression of miR-190 in adipose tissue was elevated; in the senescent cell model, the expression of miR-190 was also up-regulated; overexpression of miR-190 in adipocytes would lead to a decrease in the expression of thermogenesis genes; the key genes for thermogenesis PRDM16 is the direct target gene of miR-190; inhibiting the level of miR-190 in aging mice can help improve the energy metabolism homeostasis of aged mice. Conclusion: miR-190 may affect adipose tissue heat production by targeting PRDM16, which in turn affects energy metabolism homeostasis and accelerates aging.