MicroRNA-190调控衰老相关的脂肪能量代谢稳态
DOI:
作者:
作者单位:

1.东南大学;2.江南大学

作者简介:

通讯作者:

中图分类号:

基金项目:


MicroRNA-190 regulates aging-related fat energy metabolism homeostasis
Author:
Affiliation:

Southeast University

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探究微小RNA(microRNA,miR)-190在衰老相关的能量代谢紊乱中的作用和潜在分子机理。方法:利用实时荧光定量PCR(real-time fluorescent quantitative -polymerase chain reaction,RT-PCR),对衰老小鼠的脂肪组织中miR-190的表达水平进行检测;构建细胞衰老模型,利用RT-PCR检测脂肪细胞中miR-190的变化;利用RT-PCR检测转染miR-190的脂肪细胞中产热基因的变化;用双荧光素酶报告基因实验验证miR-190的靶基因;利用antagomir注射抑制衰老小鼠脂肪中产生miR-190,检测代谢表征。结果:衰老进程中,脂肪组织中miR-190表达升高;衰老细胞模型中,miR-190的表达同样上调;脂肪细胞中过表达miR-190会导致产热基因的表达降低;产热关键基因PRDM16是miR-190的直接靶基因;抑制衰老小鼠中miR-190的产生,可以有助于改善衰老小鼠的能量代谢稳态。结论:miR-190可能通过靶向PRDM16影响脂肪组织产热,继而影响能量代谢稳态,加速衰老。

    Abstract:

    Objective: To explore the role and potential molecular mechanism of microRNA (miR)-190 in aging-related energy metabolism disorders. Methods: Real-time fluorescent quantitative PCR (RT-PCR) was used to detect the expression level of miR-190 in the adipose tissue of aging mice; a cellular senescence model was constructed and RT-PCR was used to detect the changes of miR-190 in adipocytes; RT-PCR was used to detect the changes in thermogenesis genes in the adipocytes transfected with miR-190; the target genes of miR-190 were verified by the dual luciferase reporter gene experiment; the metabolic homeostasis was determined in aged mice injected with miR-190 antagomir. Results: During the aging process, the expression of miR-190 in adipose tissue was elevated; in the senescent cell model, the expression of miR-190 was also up-regulated; overexpression of miR-190 in adipocytes would lead to a decrease in the expression of thermogenesis genes; the key genes for thermogenesis PRDM16 is the direct target gene of miR-190; inhibiting the level of miR-190 in aging mice can help improve the energy metabolism homeostasis of aged mice. Conclusion: miR-190 may affect adipose tissue heat production by targeting PRDM16, which in turn affects energy metabolism homeostasis and accelerates aging.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2021-04-27
  • 最后修改日期:2021-07-06
  • 录用日期:2021-12-24
  • 在线发布日期:
  • 出版日期: