新生儿急性呼吸窘迫综合征患儿血浆miR-6833-3p的表达水平及诊断效能分析
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1.南京医科大学附属儿童医院;2.南京医科大学附属脑科医院

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南京市医学科技发展资金(QRX17086)


Study of the expression and diagnostic effect of miR-6833-3p in neonatal acute respiratory distress syndrome
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Nanjing Medical Science and technique Development Foundation (No.QRX17086)

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    摘要:

    目的:筛选并验证分析与新生儿急性呼吸窘迫综合征相关的microRNA,初步研究患儿血浆中miR-6833-3p表达水平及其诊断效能。方法:选取本院25例诊断新生儿急性呼吸窘迫综合征的患儿及对照组 32例为研究对象。采用微流体芯片技术筛选与新生儿急性呼吸窘迫综合征相关的microRNA,对于组间差异表达超过5倍的miRNAs进一步进行实时荧光定量聚合酶链式反应法( RT-PCR)验证芯片重复性及靶基因预测,并对两组血浆miR-6833-3p的表达水平进行检测并与急性生理评分做相关性分析,通过绘制受试者工作特征( ROC) 曲线分析 miR-6833-3p在患者中的诊断效能并计算诊断敏感度及特异度。结果:通过基因芯片筛选出24个与新生儿急性呼吸窘迫综合征相关的高表达差异基因。表达差异超过5倍的miRNAs有8个,4个上调的分别是miR-31-5p,miR-4754,miR-6833-3p和miR-192-3p。4个表达下调的基因分别是miR-362-3p,miR-11a,miR-7a-2-3p和miR-1382。通过验证发现miR-6833-3p在观察组血浆中 的表达差异最大显著上调( P<0.01)且与APACHE II评分中的急性生理评分呈正相关(R=0.731,P<0.001)。通过靶基因预测分析发现miR-6833-3p与PI3-K/Akt、MAPK信号通路可能密切相关。相关ROC曲线结果也显示,miR-6833-3p对预测新生儿急性呼吸窘迫综合征的ROC曲线下面积为 0.848,其诊断最佳阈值为 1.03,约登指数取得最大值为 0.59,此时 miR-6833-3p的诊断敏感度为 84.55% ,特异度为75.36% 。结论:miR-6833-3p在新生儿急性呼吸窘迫综合征患儿血浆中的表达水平显著升高,且对新生儿急性呼吸窘迫综合征的发生的预测具有一定的临床诊断效能,可能作为新生儿急性呼吸窘迫综合征新的特异性标志物。

    Abstract:

    Objective: To screen and verify microRNA in neonatal acute respiratory distress syndrome, preliminary study on miR-6833-3p level and its diagnostic effect in serum of neonatal acute respiratory distress syndrome. Methods: Twenty-five infants with acute respiratory distress syndrome and 32 controls were investigated in this study. miRNA microarray was utilized to explore the expression of miRNA in serum. Real-time PCR was used to verify the reproducibility of diagnostic chips and the miRNA target prediction on miRNAs that were over five folds differences between groups. Furthermore, correlation was conducted between levels of miR-6833-3p and APACHE II scores. The diagnostic effect, sensitivity, and specificity were analyzed according to ROC curve. Results: Twenty-four aberrant miRNAs were screened out as highly expressed, among these miRNAs there were 8 miRNAs with over five folds differences of expression: miR-31-5p,miR-4754,miR-6833-3p and miR-192-3p were up-expression; miR-362-3p, miR-11a,to target prediction. ROC curve showed that area under the curve was 0.848, with optimum threshold was 1.03, Youden's index was 0.59 with sensitivity 84.55% and specificity 75.36%. Conclusion: miR-6833-3p was significantly raised in neonatal acute respiratory distress syndrome. miR-6833-3p may has certain clinical diagnostic efficacy and a new biomarker as neonatal acute respiratory distress syndrome.

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  • 收稿日期:2021-05-07
  • 最后修改日期:2021-09-10
  • 录用日期:2021-11-02
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