Objective This study aimed to investigate the epidemiology, risk factors and prognosis of candidemia caused by Candida albicans and non-albicans. Methods The clinical data of 171 patients with Candidemia in the First Affiliated Hospital of Nanjing Medical University from January 2016 to December 2020 were retrospectively analyzed. Chi square test and multivariate logistic regression analysis were used to compare the risk factors of patients with bloodstream infection in Candida albicans and non-albicans group, to identify the risk factors related to Candida infection, and to further analyze the independent risk factors related to the prognosis of Candidemia. Results Candida glabrata (28.65%) was the most common isolate in candidemia patients, followed by Candida albicans (28.07%), Candida tropicalis (21.64%), Candida parapsilosis (18.13%) and other Candida (3.51%), Candida non-albicans accounted for 71.93% (123/171). Multivariate analysis showed that mechanical ventilation (OR 2.588; 95% CI: 1.119-5.987; P=0.026) was an independent risk factor for Candida albicans bloodstream infection. All Candida strains were sensitive to amphotericin B. The susceptibility rates of Candida albicans, Candida glabrata and Candida parapsilosis to fluconazole were 91.8%, 91.7% and 90.3% respectively. The drug resistance of Candida tropicalis was relatively high. 48 patients died among 171 patients with Candidemia, with a mortality rate of 28.07%. Multivariate analysis showed that age (OR 1.027; 95%CI:1.004-1.050; P=0.022), deep vein catheterization (OR 2.489; 95%CI:1.033-5.996; P=0.042) and ICU admission (OR 2.921; 95%CI:1.020-8.362; P=0.046) was an independent risk factor associated with mortality. Conclusion The distribution of Candida in our hospital is different from that in other hospitals. Most of Candida are highly sensitive to antifungal drugs, but the drug resistance rate of Candida tropicalis is relatively high. It may provide useful information for the preliminary experience of antifungal drug selection.