Abstract:Objective:To investigate the effects of adenovirus E1A associated 300 kDa protein (p300) to regulate cell migration, invasion and production of matrix metalloproteinase-2 (MMP2) on non-small cell lung cancer (NSCLC) cells upregulated by IL-17 stimulation. Methods: After NSCLC cells (H1299) incubated with IL-17, scratch and transwell experiments were performed to observe the cell migration and invasion. Then the p300 and MMP2 protein expression was detected using western blot (WB). Meantime, p300 overexpression plasmids (pcDNA3.1/p300) or short hairpin RNA (shp300) plasmids were transfected into H1299 cells (the latter was stimulated with IL-17), and the cell migration, invasion and MMP2 synthesis were again examined by the same method described above. Results:The levels of cell migration, invasion and p300 or MMP2 expression were obviously elevated in H1299 cells treated by IL-17. p300 overexpression could up-regulate the cell migration, invasion and MMP2 production, while p300 gene knockdown by shp300 in H1299 cells, the cell migration, invasion and MMP2 expression induced by IL-17 were significantly reduced. Conclusion:IL-17-upregulated p300 in H1299 cells can promote the cell migration, invasion and MMP2 production.