Objective: To investigate the production of drug antibodies in cancer patients treated with cefoperazone and meropenem, and to provide the basis for rational use of antibiotics and accurate medical treatment. Methods: Microcolumn gel method was used to detect the production of antibodies in 501 cases of cancer patients after using cefoperazone and meropenem and influencing factors were also analyzed. SPSS 22.0 was used to analyze the results of the test. Results: The positive rate of cefoperazone drug antibody in cancer patients was 30.54% (124/406), and the positive rate of 40.00% (38/95) for meropenem drug antibody. The cefoperazone and meropenem drug antibody-positive patients were mainly blood system diseases, of which the positive rates was 36.43% and 40.96% respectively. The results of different age groups showed that the positive rate of cefoperazone drug antibody was the highest in 31-40 years old group, reaching 70.37%, which was significantly higher than that of other age groups (P < 0.01). The positive rate of meropenem drug antibody was the highest in 41-50 years old group, reaching 86.67%, which was significantly higher than that of other age groups (P < 0.01). Conclusion: The use of cefoperazone and meropenem in cancer patients could induce drug antibodies and the production of drug antibodies is related to disease, sex, and age.