Objective: To analyze the clinical value of conventional MRI features in predicting IDH gene mutation in diffuse astrocytoma. Methods: 85 patients with pathologically confirmed grade Ⅱ diffuse astrocytoma (27 cases with IDH-wild type, 58case with IDH-mutant) who underwent preoperative conventional MRI examination were enrolled in this retrospective study. Two neuroradiologists who were blind to the histopathological results independently conducted the quantitative analysis of MRI features according to the VASARI (visually accessible rembrandt images) scoring system. Mann-Whitney U test, Chi-square test or Fisher's exact test was used to compare the statistical differences in imaging features between IDH-mutant group and IDH-wild group. Logistic regression analysis and receiver operating characteristic (ROC) curve analysis were performed to evaluate the diagnostic efficiency of imaging characteristics in predicting the IDH mutation of diffuse astrocytoma. Results: The two radiologists had a good agreement on the scores of conventional MRI features. Age, tumor location, ependymal invasion, deep white matter invasion, tumor crosses midline and T2/FLAIR mismatch signs are statistically significant in predicting IDH gene mutation in diffuse astrocytoma (P<0.05). Age, Tumor Location and T2/FLAIR mismatch signs were independent predictive risk factors, and the area under curve (AUC) were respectively 0.779, 0.705 and 0.840. The combination of the three parameters has the highest diagnostic efficiency in predicting IDH gene mutation in diffuse astrocytoma, with an AUC of 0.927. Conclusion: Conventional MRI features before surgery are helpful to non-invasive predict IDH gene mutation in diffuse astrocytoma.