National Natural Science Foundation of China No.81902328
目的：探讨长链非编码RNA TPTEP1在肺腺癌中的表达及其作用。方法： 收集86对肺腺癌患者的癌组织及癌旁组织样本、33对肺腺癌患者术前及术后的血浆样本,采用qRT-PCR检测TPTEP1 在所收集组织、血浆样本及肺腺癌细胞株中的表达量。采用慢病毒过表达TPTEP1在肺腺癌细胞株A549中的表达后,利用CCK-8、Tranwell实验评估肺腺癌细胞的增殖和侵袭迁移能力。结果：肺腺癌组织的TPTEP1表达量相比对照组明显降低,并且其表达量与肺腺癌的肿瘤大小,分化程度,淋巴结转移和TNM分期明显相关。肺腺癌患者术后血浆中的TPTEP1表达量较术前出现了明显的上升。过表达TPTEP1明显抑制了肺腺癌细胞株的增殖、侵袭和迁移能力。结论：TPTEP1在肺腺癌患者癌组织及术前血浆中的表达量明显降低,并且可以抑制肺腺癌细胞的增殖和转移能力,可能成为肺腺癌的诊断治疗可行的潜在靶标。
Objective: To investigate the expression and function of long non-coding RNA TPTEP1 in lung adenocarcinoma. Methods: Eighty-six pairs of lung adenocarcinoma patients tumor and adjacent-tumor tissue samples, 33 pairs of preoperative and postoperative plasma samples from lung adenocarcinoma patients were collected, and qRT-PCR was used to detect the expression of TPTEP1. The correlation between TPTEP1 levels and clinical features were analyzed. After using lentivirus to overexpress the expression of TPTEP1 in lung adenocarcinoma cell lines A549, CCK-8 and tranwell experiments were used to evaluate the proliferation, invasion and migration ability of lung adenocarcinoma cells. Results: The expression of TPTEP1 in lung adenocarcinoma tissues and cell lines was significantly lower than that in the control group, and its expression was significantly related to the tumor size, tumor differentiation, lymph metastasis and TNM stage of lung adenocarcinoma. Overexpression of TPTEP1 significantly inhibited the proliferation, invasion and migration of lung adenocarcinoma cells. Conclusion: The expression of TPTEP1 in lung adenocarcinoma tissue and preoperative plasma is significantly reduced, and it could inhibit the proliferation and metastasis of lung adenocarcinoma cells, which might provide a feasible potential target for the diagnosis and therapy of lung adenocarcinoma.