Objective: To study the relationship between thyroid hormone synthesis and metabolism-related gene expression and browning of visceral fat. Methods: The visceral fat tissues of normal weight and obese persons with normal thyroid function were collected, and the mRNA expression of genes related to thyroid hormone synthesis and metabolism, visceral fat beige functional protein and transcription factors were detected by fluorescence real-time quantitative PCR, then performed the correlation analysis. Results: The mRNA expression of thyroid hormone transporters included solute carrier family 16 members 2 and 10 (SLC16A2, SLC16A10) and solute carrier organic anion transporter family member 1C1 (SLCO1C1), Among them, the difference of SLC16A2 is statistically significant. Thyroid stimulating hormone β subunit (TSHB) and iodothyronine deiodinase 2 (DIO2) decreased significantly in the visceral adipose tissue of obese people. The expression of visceral fat beige functional protein UCP1 and marker transcription factor PR domain-containing 16 (PR domain-containing 16, PRDM16) are positively correlated with the expression of the above genes after adjusted by age, gender, body mass index and FT3. Conclusion: Decreased beige function of visceral fat in obese people is closely related to thyroid hormone synthesis and metabolism.