Objective: Enterohemorrhagic E.coli O157:H7 (EHEC O157:H7) is a common foodborne pathogenic microorganism, which mainly causes foodborne diseases. It is very important to develop EHEC O157:H7 rapid detection methods and establish a new method reserve for EHEC O157:H7 detection. Methods: Two EHEC O157:H7 detection methods were established and optimized based on the conserved region sequence of EHEC O157:H7 combined with Recombinase-Aided Amplification (RAA), fluorescence quantitative and Lateral Flow Dipstick (LFD), then the sensitivity and specificity of the two methods were evaluated. Results: Both detection methods can be completed within 16min at 39℃, and can detect 104 copies, showing high sensitivity. The two methods did not cross react with plasmid templates of other common intestinal pathogens (Staphylococcus aureus, Salmonella, Campylobacter, Yersinia, Shigella) and showed good specificity. Conclusion: The two EHEC O157:H7 detection methods established in this study have short detection time, good specificity, sensitivity and repeatability, and can be used for rapid detection of EHEC O157:H7.