Endometriosis (EMS) has been recognized in recent years as a fibrotic disease, and the fibrosis process of EMS lesions is strongly associated with EMS-related pain, infertility and drug resistance. Myofibroblasts (MFBs) are the key cells of EMS fibrosis, MFBs have a variety of cell sources in eutopic lesions and many activation pathways. Inhibiting MFBs activation,promoting apoptosis or cell senescence can inhibit tissue fibrosis. In this paper, the research progress about the correlation between fibrosis and clinical manifestations of EMS, the sources and activation pathways of MFBs in EMS, and targeting MFBs to inhibit fibrosis are reviewed.