Objective;To study the expression and correlations of PTTG,PTEN and clinicopathological factors.Methods:In s itu hybridization and PV-9000 Polymer Detection System were used to detect the expression of PTG and PTEN in 71 cases of breast carcinomas (BRCA). Results; Tumor tissue expressed a significantly higher level of PTG and lower level of PTEN at mRNA or protein than the corresponding normal tissue (P<0.05), over-expression PTTG protein in BRCA correlated with lower-differentiation and lymph node metastasis. There were lots of correlations in BRCA between lower-expression of PTEN protein and the tumors'pectoralis invasion, higher pathological grade, and pathologic classification. The expression of PTTG was not correlated with that of PTEN at mRNA or protein level in BRCA. Conclusion: Over-expression of P1TG and lower-expression of PTEN may be related to human breast carcinogenesis.