高糖诱导的MAPK激活对人肾小球系膜细胞的影响
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Effect of MAPK activation in response to high glucose on human glomerular mesangial cells
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    目的:探讨高糖对人肾小球系膜细胞丝裂原活化蛋白激酶(MAPK)活性的影响及MAPK在人肾小球系膜细胞的纤维结合蛋白(FN)分泌和c-fos表达中的作用.方法:采用人肾小球系膜细胞进行体外培养,高糖作为刺激因素,PD98059作为MAPK特异性抑制剂,同位素示踪法测定MAPK活性,ELISA法测定培养上清中FN含量,免疫细胞化学方法检测c-fos的表达.结果:高糖可增加MAPK活性、促进FN分泌、增加c-fos的表达.抑制MAPK后,可阻止高糖诱导的FN分泌以及c-fos表达.结论:高糖可激活人肾小球系膜细胞内的MAPK,促进FN的分泌,c-fos的表达及导致糖尿病肾病的发生.

    Abstract:

    Objective:To investigate the activity and possible role of MAPK in mesangial cells in response to high glucose and to research the mechanism of diabetes nephopathy. Methods:The human mesangial cells were cultured and stimulated by high glucose.The MAPK activity was measured by liquid scintilation counting. Production of fibronectin was analyzed by ELISA. The expression of c-fos was assayed by immunochemistry. Results: Compared with the control glucose, high glucose increased the activity of MAPK, the expression of c-fos and the production of FN. When PD98059 were used, these three all decreased. Conclusion: High glucose can active MAPK, increase the expression of c-fos and the production of FN and contribute to diabetes nephopathy.

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高景燕.高糖诱导的MAPK激活对人肾小球系膜细胞的影响[J].南京医科大学学报(自然科学版),2005,(1):22-24

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  • 收稿日期:2004-04-01
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