Objective:To summarize the clinical data and investigate the treatment of patients with prematuer ovarian failure.Methods: Thirty-one patients with premature ovarian failure were evaluated from January 1999 to December 2003. Results: Among 31 cases, there were 22 infertile patients and 9 patients with fertility, The average period of amenorrhea was 2.8 years(from 5 months to 7 years),in which there were 7 cases with the abrupt cessation of menstruation and 24 patients with oligomenorrhea and gradually to cessation of menstruation . 16 patients had the symptoms of estrogen deficiency and 48.4% of patients had genital and breast atrophy at different rates. The circulating concentration of estradiol was reduced and follicle-stimulating hormone and luteinizing hormone levels increased. All patients were treated with a combination of estrogen and progesterone. The cure rate of premature ovarian failure was 22.6% and no patient was treated to have a pregnancy. Conclusion: Premature ovarian failure is a disease of multifactors. Further study and emphasis ought to be taken to investigate it.