文章摘要
成 峰,王学浩,钱晓峰,王 科,黄 蓬.冷保存时间对部分肝移植肝再生影响的实验研究[J].南京医科大学学报,2006,(11):1035~
冷保存时间对部分肝移植肝再生影响的实验研究
Experiment study of influence of cold ischemia on liver regeneration following partial liver transplantation
投稿时间:2006-04-28  
DOI:10.7655
中文关键词: 部分肝移植  肝再生  肿瘤坏死因子-α  白细胞介素-6
英文关键词: 
基金项目:
作者单位
成 峰 南京医科大学第一附属医院肝移植中心江苏 南京 210029 
王学浩  
钱晓峰  
王 科  
黄 蓬  
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中文摘要:
      目的:建立大鼠部分肝移植模型基础上,研究不同冷保存时间对移植肝再生相关细胞因子表达的影响。方法: 实验分为:冷保存30 mim部分肝移植组、冷保存4 h部分肝移植组和冷保存10 h部分肝移植组,观察各组生存率并分别于术后1、2、4天取肝组织,免疫组化检测各组增殖细胞核抗原(PCNA)、TNF-α、IL-6的表达。结果:冷保存30 mim部分肝移植组、冷保存4 h部分肝移植组和冷保存10 h部分肝移植组存活率分别为79%、71%、29%,和其他实验组相比:冷保存10 h部分肝移植组PCNA、TNF-α、IL-6表达降低(P < 0.05)。 结论:随着冷保存时间的延长,降低了部分肝移植术后的肝再生能力和生存率。冷保存时间影响移植肝TNF-α和IL-6的表达,移植物中细胞因子TNF-α和IL-6的表达对移植肝肝再生过程中起重要的调控作用。
英文摘要:
      Objective: To evaluate the effects of cold preservation on hepatocyte proliferation in a novel model of partial liver transplantation in rats. Methods: Donor livers were preserved at 0-4℃ for 30 minutes, 4 hours and 10 hours in University of Wisconsin solution. The expression of the proliferative cell nuclear antigen(PCNA), necrosis factor alpha(TNF-alpha) and interleukin 6(IL-6) of liver in rats were evaluated by immune histochemical method 1,2,4 d after operation, respectively. Results:30-minute, 4-hour and 10-hour preservation before 50% partial transplantation resulted in 79%, 71% and 29% animal survival, respectively. Prolonged time of cold preservation(10 h) was associated with a dramatic decrease of the marker of regeneration(P < 0.05). TNF-alpha and IL-6 was significantly decreased in recipient rats compared with the other groups. Conclusion:Periods of cold preservation are significantly associated with the regenerative ability of the liver and animal survival. Cold preservation decreases the expression of TNF-alpha and IL-6 production in graft. They play a primary role in modulating hepatocyte proliferation in the cold ischemic liver.
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