Objectives: To explore the role of epidermal growth factor receptor（ＥＧＦＲ） on UV-induced VEGF secretion of MEF. Methods: Flow cytometry was used to detect total receptor and tyrosine-phosphorylated EGFR, respectively. the concentration of VEGF was determined by ELISA. Results: EGFR phosphorylation was detectable within 15 min after UV exposure. The level of EGFR phosphorylation reached peak 30 min after UV exposure, and was decreased to basal levels 2 to 8 h after UV exposure. VEGF expression was not increased in EGFR（-／-） MEF following UV exposure. In contrast VEGF expression, was significantly increased in egfr（+／+） MEF after UV exposure. PD153035 inhibited the induction of VEGF protein expression in UVB-treated cells in a dose-dependent manner. Conclusion: EGFR plays an important role in UV-induced VEGF secretion in MEF.