文章摘要
蒋 冬,丁 炯,金国华,田美玲,秦建兵.银杏内酯对大鼠胚基底前脑NOS、AchE阳性神经元发育的影响[J].南京医科大学学报,2006,(12):1187~1191
银杏内酯对大鼠胚基底前脑NOS、AchE阳性神经元发育的影响
Effects of ginkgolide on the development of NOS and AchE positive neurons in the embryonic basal forebrain
投稿时间:2006-04-18  
DOI:10.7655
中文关键词: 银杏内酯  胚基底前脑; NOS阳性神经元; AchE阳性神经元; 细胞培养; 图像分析; 大鼠
英文关键词: ginkgolide  embryonic basal forebrain  NOS positive neurons  AchE positive neurons  cell culture  image analysis  rat
基金项目:
作者单位
蒋 冬 南京医科大学解剖学系江苏 南京 210029 
丁 炯 南京医科大学解剖学系江苏 南京 210029 
金国华 南通大学基础医学院江苏 南通 226001 
田美玲 南通大学基础医学院江苏 南通 226001 
秦建兵 南通大学基础医学院江苏 南通 226001 
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中文摘要:
      目的:探讨银杏内酯对大鼠胚基底前脑NOS、AchE阳性神经元发育的影响及可能的作用机制。方法: 实验分银杏组、神经生长因子(NGF)组、脑原神经营养因子(BDNF)组和单纯对照组。取孕17天SD大鼠胚基底前脑原基制成细胞悬液接种于96孔培养板和2块24孔培养板中,分别加入含银杏内酯、NGF、BDNF及不含上述成分的DMEM培养液。于体外培养18天后,96孔培养板行MTT比色分析测定光密度值(A值),以检测培养的神经元活力。2块24孔培养板分别行NADPH-d和AchE组织化学染色,显微镜下计数各组每孔中一氧化氢合酶(NOS)、AchE阳性神经元数目,并用CMM-301图像分析系统对两种神经元的细胞面积和细胞周长进行处理,数据用方差分析、SNK检验作统计学处理。结果:银杏组MTT比色分析的A值和NOS、AchE阳性神经元数目、细胞面积、细胞周长等指标均优于单纯对照组,达到或仅稍差于NGF组或BDNF组的指标。结论:银杏内酯在促进大鼠胚基底前脑NOS、AchE阳性神经元发育方面具有类似NGF或BDNF的作用。
英文摘要:
      Objective:To study the effects of ginkgolide on the development of NOS and AchE positive neurons in the embryonic basal forebrain. Methods:The rats were divided into Ginkgo, NGF, BDNF and control groups. The cell suspension of embryonic basal forebrain was prepared from E17-day old SD rats. Dissociated cells were plated into 96-well and two 24-well cultured plates which had DMEM medium containing ginkgolide, NGF, BDNF respectively. Eighteen days after culturing, MTT staining analysis was performed to test the vitality of the cultured neurons in the 96-well cultured plate. The cultured neurons in two 24-well plates were detected by using NADPH-d and AchE histochemical stains respectively. The numbers of NOS and AchE positive neurons were counted under microscope. The cell areas and circumferences of NOS and AchE positive neurons were analyzed with CMM-301 computer image system. The data were statistically treated with ANOVA and SNK test. Results:The optical density of MTT staining analysis, the number, size and circumference of NOS and AchE positive neurons of ginkgolided treated rats were higher than those of the rats of the control group and similar to those of the NGF and BDNF group. Conclusion:Ginkgolide has similar effects of the neurotrophins such as NGF and BDNF in promoting the development of NOS and AchE positive neurons in the embryonic basal forebrain.
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