文章摘要
王国柱,吴文溪,张玉华.大鼠结肠癌腹腔转移模型的建立和肿瘤切除术后结肠吻合口羟脯氨酸含量的测定[J].南京医科大学学报,2006,(12):1255~
大鼠结肠癌腹腔转移模型的建立和肿瘤切除术后结肠吻合口羟脯氨酸含量的测定
Development of abdominal transfered colon carcinoma rats model and the concentrations of hydroxyproline in colon stoma tissue after tumorectomy
投稿时间:2006-07-15  
DOI:10.7655
中文关键词: 结肠癌  动物模型  羟脯氨酸
英文关键词: Colon Carcinoma  Rats Model  Hydroxyproline
基金项目:
作者单位
王国柱 南京医科大学第一附属医院普外科江苏 南京 210029 
吴文溪  
张玉华  
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中文摘要:
      目的:建立一种新型的大鼠结肠癌腹腔转移模型,通过测定吻合口组织羟脯氨酸含量研究荷瘤大鼠行肿瘤切除术后吻合口的愈合情况。方法:人结肠腺癌细胞SW-480注射种植到裸鼠皮下,形成瘤体后将瘤块开腹移植到SD大鼠结肠黏膜下造成6例大鼠结肠癌腹腔转移模型,行肿瘤切除术。选取6只等体重SD大鼠为对照组,进行假手术。1周后测量各组吻合口抗张力,用高效液相法(HPLC)测量吻合口组织羟脯氨酸含量。结果:SD大鼠成瘤率为60%,并且成瘤大鼠均可见腹腔转移。荷瘤组吻合口张力和羟脯氨酸含量均显著低于假手术组。结论:该方法制作对模型稳定可靠,而肿瘤可显著延迟吻合口愈合。
英文摘要:
      Objective:To develop a new kind of abdominal transfered colon carcinoma model in rats, and to evaluate the healing of stoma by measure the concentrations of hydroxyproline in tissue of colon stoma. Methods:Colon Carcinoma cell SW480 was inoculated subcutaneously into athymic mice to develop an implantation tumor,Subsequently the tumor lumps were implanted to the SD rats’ colon mucosa to setup the model. The tumor tissues were cut and the colons were anastomosed. 6 rats which were not implanted tumors but colons been cut and anastomosed were included into control group. Then measure the tensile strength and concentrations of hydroxyproline of colon stoma tissue with HPLC. Results:60% rats were success to establish abdominal transfered Colon Carcinoma models. The tensile strength and the concentrations of hydroxyproline in colon stoma tissue were both lower in experiment group than in control group(P<0.05.) Conclusion:It’s a reliable method to build tumor model and tumor significantly delays the healing of stoma.
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