文章摘要
裴凌鹏,董福慧,惠伯棣,朱 佳.角黄素改善H2O2对成骨细胞损伤的影响[J].南京医科大学学报,2007,(2):113~117
角黄素改善H2O2对成骨细胞损伤的影响
Effects of canthaxanthin on H2O2-induced damage of osteoblast
投稿时间:2006-06-19  
DOI:10.7655
中文关键词: 成骨细胞  超氧化物歧化酶  流动性  角黄素
英文关键词: osteoblast  SOD  fluidity  canthaxanthin
基金项目:国家自然科学基金资助项目(NO.30171169)
作者单位
裴凌鹏 中国中医科学院骨伤科研究所北京 100700 
董福慧 中国中医科学院骨伤科研究所北京 100700 
惠伯棣 北京联合大学应用文理学院北京 100083 
朱 佳 中国中医科学院骨伤科研究所药理实验室北京 100700 
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中文摘要:
      目的:在H2O2胁迫下,研究角黄素增强成骨细胞抗自由基损伤的作用。方法:用H2O2作用M3T3-E1成骨细胞,建立自由基损伤细胞模型。培养的成骨细胞分为对照组、模型组、角黄素低剂量组(1 × 10-8 mol/L)、角黄素中剂量组(1 × 10-7 mol/L)和角黄素高剂量组(1 × 10-6 mol/L)。测定不同处理组细胞活力、超氧化物歧化酶(superoxide dismutase, SOD)活性、活性自由氧(reactive oxygen species, ROS)含量、脂质过氧化物(lipid oxygen, LPO)含量,同时利用荧光偏振法测定细胞膜流动性。结果: 模型组细胞活力、SOD活性、细胞膜流动性均比不同角黄素组有显著降低(P﹤0.01),而ROS含量、LPO含量均比不同角黄素组有显著升高(P﹤0.01)。结论:H2O2摄入会导致大鼠成骨细胞的氧化损伤,而角黄素可以预防或降低此类损伤对细胞的影响。
英文摘要:
      Objective:To investigate the effects of canthaxanthin on the anti-oxidative damage function of osteoblast. Methods: M3T3-E1 osteoblasts were randomly divided into five groups, including control group,model group,canthaxanthin groups[low-dose(1×10-8 mol/L),middle-dose(1 × 10-7 mol/L),high-dose(1 × 10-6 mol/L)]. The activity of cells, superoxide dismutase(SOD), the content of reactive oxygen species(ROS), lipid oxygen(LPO) and membrane fluidity were tested and compared. Results:Compared with canthaxanthin groups,the activity of cells, SOD activity and membrane fluidity in the model group were significantly decreased(P﹤0.01). However, the content of ROS and LPO were significantly increased(P﹤0.01). Conclusion:H2O2 can induce oxidative damage of M3T3-E1 osteoblasts, but canthaxanthin can prevent and decrease its influence.
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