文章摘要
刘兵荣,张 勇,王京娥,丁新生.实验性脑出血后血肿周围细胞凋亡及黄芪多糖的干预作用[J].南京医科大学学报,2007,(2):153~157
实验性脑出血后血肿周围细胞凋亡及黄芪多糖的干预作用
Variety of apoptosis after experimental intracerebral hemorrhage and effects of astragalus polysaccharide
投稿时间:2006-07-10  
DOI:10.7655
中文关键词: 脑出血  凋亡  黄芪多糖  NF-κB  超微结构
英文关键词: intracerebral hemorrhage  apoptosis  astragalus polysaccharide  NF-κB  ultrastructure
基金项目:
作者单位
刘兵荣 南京医科大学第一附属医院神经内科江苏 南京 210029 
张 勇  
王京娥  
丁新生  
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中文摘要:
      目的:探讨黄芪多糖(astragalus polysaccharide,APS)对大鼠脑出血(intracerebral hemorrhage,ICH)后神经功能缺损、细胞凋亡及核因子-κB(nuclear factor-kappaB,NF-κB)表达的影响。方法:雄性SD大鼠分为正常组、假手术组、模型组与治疗组。模型组与治疗组均采用Ⅶ型胶原酶诱导脑出血模型,假手术组以等量生理盐水代替Ⅶ型胶原酶,治疗组给予黄芪多糖25 mg/kg腹腔注射,每天一次。分别于6 h和1、3、5天观察大鼠神经行为学评分,用凋亡原位末端标记技术(TUNEL)和免疫组化方法检测血肿周围神经凋亡和NF-κB表达的动态变化。透射电镜观察术后3天血肿周围神经元超微结构的变化。结果:与模型组比较,黄芪多糖干预后 3天和5天大鼠神经行为学评分明显减少(P < 0.05),6 h、1、3、5天时NF-κB阳性细胞数明显减少(6 h、1天 P < 0.05,3、5天P < 0.01),TUNEL阳性细胞数1、3、5天时明显减少(1、3天比较P < 0.05,5天P < 0.01)。神经元超微结构改变亦比模型组减轻。结论: ICH后血肿周围神经细胞损伤有凋亡机制参与,黄芪多糖可以有效减轻脑出血后的细胞凋亡,其机制可能与抑制NF-κB表达有关。
英文摘要:
      Objective:To explore the effects of astragalus polysaccharide on neurological function deficit and neuron apoptosis and expression of NF-κB(nuclear factor-kappa B) in rats after interacerebral hemorrhage(ICH). Methods:All rats were randomly divided into normal group, sham-operation group, model group and treatment group. ICH model was established by injection of collagenaseⅦ. Sham-operation group was established by saline instead.Treatment group was injected with astragalus polysaccharide(25 mg/kg) through celiac once a day. At 6 hour and on the 1st day, 3rd day and 5th day, the neurological behaviors of rats were observed .By the methods of TUNEL and immunohistochemisty, the dynamic changes of apoptosis and the expression of NF-κB in the tissue surrounding hematoma in were detected ICH rats. Ultrastructures of the cells around the hematoma were observed with transmission electron microscope. Results:Contrast to model group, the score of neurological behavior in treatment group rats was decreased significantly on 3rd and 5th day(P < 0.05), as well as TUNEL positive cells(P < 0.05 and P < 0.01). Significantly difference of NF-κB positive cell between model group and treatment group was found on the first, 3rd and 5th day(P < 0.05 and P < 0.01). The pathological changes of the treatment group were milder than those of the model group 3 days after operation. Conclusion:Apotosis is a pattern of death after intracerebral hemorrhage,and the expression of NF-κB is an important step in apoptotic cell death after ICH. Astragalus polysaccharide can effectively alleviate apoptosis after ICH. Preventing NF-κB expression may be involved in the treatment.
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