Objective：To evaluate inter-ventricular myocardial synchrony in normal subjects by velocity vector imaging（VVI）. Methods：Siemens Sequoia 512 equipment with 4V1c probe and VVI sofeware were used. Thirty-five subjects were enrolled in this study. Results：The fifty percent of time from R wave to peak of S wave in apical segments of anterior septum， inferior septum and anterior wall were all longer than those in basal and mid segments（P ＜ 0.05）. In parasternal short-axis view at level of mitral valve，there were significant differences in the seventy-five percent of time from R wave to peak of S wave among inferior wall， anterior wall, anterior septum and lateral wall，as well as in fifty percent of time from R wave to peak of S wave. Conclusion：It is important to maintain myocardial synchrony for the cardiac function. VVI can noninvasively， quantitatively， accurately and quickly assess myocardial synchrony.