文章摘要
王新河,王 斌,钱燕宁.短暂性局灶性脑缺血再灌注后氯化锂对大鼠海马CA1区神经元凋亡及糖原合成酶激酶-3β的影响[J].南京医科大学学报,2007,(5):407~410
短暂性局灶性脑缺血再灌注后氯化锂对大鼠海马CA1区神经元凋亡及糖原合成酶激酶-3β的影响
Effects of lithium chloride on neuronal apoptosis and expression of glycogen synthase kinase(GSK)-3beta of hippocampal CA1 region after transient focal cerebral ischemia/reperfusion in rats
投稿时间:2006-10-10  
DOI:10.7655
中文关键词: 脑缺血  细胞凋亡  氯化锂  GSK-3β  大脑中动脉闭塞
英文关键词: brain ischemia  apoptosis  lithium chloride  GSK-3β  middle cerebral artery occlusion
基金项目:国家人事部出国留学人员基金资助项目(ZA0001),南京医科大学创新基金资助项目(MC0005)
作者单位
王新河 南京医科大学第一附属医院麻醉科江苏 南京 210029 
王 斌 南京医科大学药理学教研室江苏 南京 210029 
钱燕宁 南京医科大学第一附属医院麻醉科江苏 南京 210029 
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中文摘要:
      目的:观察氯化锂对短暂局灶性脑缺血再灌注大鼠海马CA1区神经元凋亡及GSK-3β的影响。方法:SD大鼠126只,随机分为正常(NO)组、假手术(SH)组、缺血再灌注(IR)组、氯化锂1(Li1)组、氯化锂2(Li2)组、氯化锂3(Li3)组。Hoechst染色观察海马CA1区神经元凋亡的变化,免疫荧光染色观察海马CA1区GSK-3β及p-GSK-3β(Ser9)的变化。结果:与SH组比较IR组细胞凋亡明显(P < 0.01)。与IR组比较各氯化锂组凋亡细胞明显减少(P < 0.05或P < 0.01) ,氯化锂剂量越大,凋亡细胞减少越明显(P < 0.05或P < 0.01)。各组GSK-3β阳性反应颗粒数差异无显著性。与SH组相比IR组p-GSK-3β(Ser9)明显增多(P < 0.01)。与IR组比较各氯化锂组p-GSK-3β(Ser9)明显增多(P < 0.01),氯化锂剂量越大,增多越明显(P < 0.05或P < 0.01)。结论:氯化锂能剂量依赖性的减轻大鼠短暂性局灶性脑缺血后海马CA1区神经细胞凋亡,增加海马CA1区p-GSK-3β(Ser9)表达。
英文摘要:
      Objective:To observe the effect of lithium chloride on neuronal apoptosis and GSK-3β of hippocampal CA1 area after transient focal cerebral ischemia/reperfusion in rats. Methods:126 male SD rats were randomly divided into normal group(NO group),sham operation group(SH group),ischemia/reperfusion group(IR group), Li1 group, Li2 group,and Li3 group. The change of neuronal apoptosis in the CA 1 region was examined by Hoechst staining. The expression and distribution of GSK-3β and p-GSK-3β(Ser9) in the cell was examined by immunofluorescence analysis. Results:The apoptotic nuclei could be detected significantly in IR groups(compared with SH group, P < 0.01). Compared to IR group, Li groups dose dependently(1~3 mmol/kg) reduced the apoptotic nuclei in happocampal CA1 area. There was no visible difference among the total amounts of GSK-3β in each group(P > 0.05). P-GSK-3β(Ser9)increased significantly after reperfusion(P < 0.01). Lithium chloride treatment can dose dependently increase the expression of p-GSK-3β(Ser9)(compared with IR groups, P < 0.01). Conclusion:Lithium chloride can dose dependently suppress neuronal apoptosis and GSK-3β expression after MCAO/ reperfusion.
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