文章摘要
刘凌翔,朱华云,张锦英,葛红梅,王同杉,刘平.两种铂类药物对人胃癌细胞Livin基因表达的影响[J].南京医科大学学报,2007,(5):415~418435
两种铂类药物对人胃癌细胞Livin基因表达的影响
Effects of two platinum compounds on the Livin gene expression of gastric cancer
投稿时间:2006-10-23  
DOI:10.7655
中文关键词: 奥沙利铂  顺铂  Livin基因  凋亡抑制蛋白  胃癌细胞
英文关键词: oxaliplatin(L-OHP)  cisplatin(CDDP)  livin  IAPs  gastric cancer cell
基金项目:江苏省“135”医学重点人才(RC2001-052)
作者单位
刘凌翔 南京医科大学第一附属医院肿瘤科江苏 南京 210029 
朱华云  
张锦英  
葛红梅  
王同杉  
刘平  
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中文摘要:
      目的:研究奥沙利铂(L?鄄OHP)或顺铂(CDDP)对人胃癌细胞株BGC?鄄823生长的抑制作用以及Livin基因表达的变化。方法:应用MTT比色法检测细胞24、48、72 h的增殖抑制率,选择3个时间段的不同浓度(IC10、IC30、IC50)铂类药物作用于BGC?鄄823细胞,RT?鄄PCR法检测Livin基因表达,Western?鄄blots法检测Livin蛋白表达量。结果:两种铂类药物作用后,BGC?鄄823的增殖受到抑制,呈时间和剂量依赖性,L?鄄OHP的量效变化更明显。两种铂类药物早期均可抑制Livin表达,CDDP作用细胞72 h后Livin表达量开始增加,而L-OHP组仍呈下降趋势。结论:L-OHP是Livin的潜在抑制剂,其对BGC-823的增殖抑制量效变化比CDDP更明显,两者对Livin表达的不同影响将为探讨Livin的调控机制和功能研究提供一定依据。
英文摘要:
      Objective:To investigate the growth inhibition of Oxaliplatin(L-OHP) or Cisplatin(CDDP) in vitro and Livin gene expression induced by the two platinum compounds on human gastric cancer cell line(BGC-823). Methods:The proliferation inhibition ratio was evaluated by MTT in the time of 24, 48 and 72 hours. Different inhibiting concentrations(IC10, IC30 and IC50) of the two platinum compounds were added into the medium of BGC-823 cells. The expression of Livin gene was detected by RT-PCR, and the protein of Livin was assessed by Western-blot. Results:The proliferation of BGC-823 was inhibited in a dose-and time-dependent manner after the treatment of CDDP or L-OHP, especially the latter was dose-dependent significantly than the former. The expression of Livin, which was down-regulated by the two platinum compounds in the earlier period, was up-regulated after 72 hours with CDDP other than L-OHP. Conclusion:L-OHP, which can more significantly inhibit the proliferation of BGC-823 in a dose-dependent manner than CDDP, is a potent inhibitor of Livin. The different influences on the expression of Livin will contribute to the investigation of regulation and function on Livin.
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