文章摘要
朱利群,徐 珊,罗 莉,李 卓,仇黎丽,徐昌芬.马鞭草有效成分对人绒毛膜癌耐药细胞株JAR/VP16的逆转作用研究[J].南京医科大学学报,2007,(5):419~423
马鞭草有效成分对人绒毛膜癌耐药细胞株JAR/VP16的逆转作用研究
Reversal of multidrug resistance in drug-resistance human choriocarcinoma cell line JAR/VP16 by effective component of Verbena Officinalis L
投稿时间:2006-09-05  
DOI:10.7655
中文关键词: 4’-甲醚-黄芩素  绒毛膜癌  多药耐药  逆转  细胞凋亡
英文关键词: 4’-methery-scutellarein  choriocarcinoma  multidrug resistance  reversal  cell apoptosis
基金项目:江苏省自然科学基金(BK2003104),南京医科大学科技创新基金(CX2002002)资助项目,江苏省高校基金自然科学基金(06KJB360067)
作者单位
朱利群 南京医科大学生殖医学实验室江苏 南京 210029 
徐 珊  
罗 莉  
李 卓  
仇黎丽  
徐昌芬  
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中文摘要:
      目的:研究马鞭草有效成分4’-甲醚-黄芩素(4’-methylether-scutellarein, 4’-M-S)对人绒毛膜癌多药耐药细胞株JAR/VP16的耐药逆转作用。方法:MTT法检测4’-M-S对JAR/VP16细胞的增殖抑制作用,确定药物逆转浓度及其对耐药细胞的逆转倍数和逆转效率;单克隆抗体酶免定量法测定耐药细胞中绒毛膜促性腺激素(hCG)分泌变化;流式细胞术检测细胞周期分布及细胞凋亡率;光镜和透射电镜观察耐药细胞形态学改变。结果:4’-M-S对JAR/VP16细胞具有明显增殖抑制作用,20 ?滋g/ml 4’-M-S可显著逆转JAR/VP16细胞对鬼臼乙叉甙(VP16)、氨甲喋呤(MTX)及放线菌素D(ACTD)的耐受性,逆转倍数为:5.02、3.67、2.48;逆转效率为:81.19%、76.89%、64.08%;4’-M-S联合作用后,耐药细胞hCG分泌量明显下降;细胞周期阻滞于G0~G1期和G2~M期,细胞凋亡率达19.71%;透射电镜观察发现细胞质空泡化、内质网扩张、核物质浓缩边聚等早期凋亡现象。结论:4’-M-S对JAR/VP16细胞具有耐药逆转作用,可能的机制是抑制耐药细胞生长及诱导细胞凋亡。
英文摘要:
      Objective:To investigate the reversal effect of the effective component of Verbena Officinalis L. (4’-methery-scutellarein,4’-M-S) on multidrug resistant JAR/VP16 cell line. Methods:MTT[3-(4,5-dimethylthiazol-2-yl) 2,5-dipheny tetrasodium bromide] assay was used to examine the action of 4’-M-S on proliferation of JAR/VP16 cells, determine the available reversal concentration of 4’-M-S and measure the reversal index and ratio on JAR/VP16 cells. Secretory volume of chorionic gonadotrophic hormone(hCG) was measured by monoclonal antibody enzyme-linked immunoquantitation. Cell cycle profile and apoptotic cell rate was examined by flow cytometry(FCM), the morphologic and ultramicrostructural changes were observed with light and transmission electron-microscope(TEM). Results:The resistance of JAR/VP16 to VP16, MTX and ACTD, was enhanced up to 5.02,3.67,2.48 folds and ratios of 81.19%,76.89%,64.08% after exposure of the cells to 20 ?滋g/ml 4’-M-S, respectively. Secretory volume of hCG in drug-resistance cell was decreased obviously when treated with combination of 4’-M-S and VP16. Cell cycle was blocked at the period of G0-G1 and G2-M , cell apoptotic rate was increased to 19.71%. The earlier apoptotic changes were observed under TEM in the cells treated. Conclusion:4’-M-S has a strong reversal effect on multidrug resistance in JAR/VP16 cells, which may be achieved by inhibiting growth of JAR/VP16 cells and inducing cell apoptosis.
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