文章摘要
沈美萍,武正炎,侯大卫,刘翠萍.三苯氧胺抑制雌激素受体阴性的甲状腺癌生长[J].南京医科大学学报,2007,(5):467~471
三苯氧胺抑制雌激素受体阴性的甲状腺癌生长
Tamoxifen inhibits the growth of ER negative thyroid carcinomas in vitro and in vivo
投稿时间:2006-12-19  
DOI:10.7655
中文关键词: TAM  甲状腺癌  细胞周期  体内试验  体外实验
英文关键词: Tamoxifen  thyroid carcinoma  cell cycle  in vitro experiment  in vivo experiment
基金项目:江苏省教育厅高校自然科学研究计划项目资助(02KB320004)
作者单位
沈美萍 南京医科大学第一附属医院普外科江苏 南京 210029 
武正炎 南京医科大学第一附属医院普外科江苏 南京 210029 
侯大卫 南京医科大学第一附属医院普外科江苏 南京 210029 
刘翠萍 南京医科大学第一附属医院内分泌科江苏 南京 210029 
摘要点击次数: 1093
全文下载次数: 92
中文摘要:
      目的:研究三苯氧胺(tamoxifene,TAM)对雌激素受体阴性的甲状腺乳头状癌和滤泡状癌的体内外作用,并初步探索其作用机制。方法:①体外实验:4-甲基偶氮四唑蓝(MTT)细胞增殖试验观察TAM对甲状腺乳头状癌细胞(IHH-4)和甲状腺滤泡状癌细胞(FTC3-133)生长的影响,流式细胞仪DNA定量法观察TAM作用后甲状腺癌细胞周期的变化和晚期凋亡的发生。免疫组织化学法和RT-PCR的方法检测2株细胞中雌激素受体(ER)的表达。②体内实验:5周龄BALB/c雌性裸鼠皮下接种甲状腺乳头状癌细胞IHH-4,肿瘤生长后,处理组予TAM 5 mg/(kg·d)灌胃,对照组予等体积安慰剂,连续5周观察肿瘤体积的生长和小鼠体重的变化,并最终比较两组间肿瘤的重量。结果:①体外实验:免疫组化法和RT-PCR法检测IHH-4和FTC-133细胞中雌激素受体均为阴性;TAM明显抑制两株甲状腺癌细胞增殖,呈时间和剂量依赖性;TAM使两株甲状腺癌细胞阻滞于G1期,并且诱导FTC3-133细胞的凋亡。②体内实验:TAM抑制甲状腺癌细胞种植瘤的生长。结论:TAM抑制甲状腺癌生长,并通过非依赖雌激素受体途径发挥作用。
英文摘要:
      Objectives:To investigate the effects of tamoxifen(TAM) on the growth of estrogen receptor(ER) negative thyroid carcinomas and the mechanisms involved. Methods:In vitro experiments, papillary thyroid carcinoma cells(IHH-4) and follicular thyroid carcinoma cells(FTC-133) were treated with tamoxifen in different doses and different lengths of time, using the dimethylthiazol-dipheltetrazolium bromide(MTT) method to observe the cell proliferation. Cell cycle and apoptosis were analysed with flow cytometric DNA studies. In vivo experiments the papillary thyroid carcinoma cells were injected into the nude mice subcutaneously, and the mice had been feed with TAM[5 mg/(kg·d)] daily since the second week. The tumor volumes were calculated weekly. Finally the mice were sacrificed and the tumors were weighed separately. Results:In vitro,TAM inhibited the proliferation of the IHH-4 and FTC-133 cell lines in dose-dependent and time-dependent pattern. TAM inhibited the growth of the IHH-4 and FTC-133 cell lines by blocking the cell cycle in G1 phase. Immunohistochemistry and RT-PCR studies showed the ER negative in both cell lines. In vitro,TAM also inhibited the growth of IHH-4 xenografts in nude mice. Conclusions:TAM inhibits the growth of thyroid carcinoma in vitro and in vivo. TAM acts independent of estrogen receptor and could be a potential choice for the treatment of the aggressive thyroid carcinomas clinically.
查看全文   查看/发表评论  下载PDF阅读器
关闭