文章摘要
文惯宇,赵德育,田 曼.RANTES启动子-403G/A基因多态性与呼吸道合胞病毒致毛细支气管炎的相关性研究[J].南京医科大学学报,2007,(5):487~490
RANTES启动子-403G/A基因多态性与呼吸道合胞病毒致毛细支气管炎的相关性研究
The association of the RANTES gene promoter -403G/A polymorphism with respiratory syncytial virus bronchiolitis
投稿时间:2006-11-20  
DOI:10.7655
中文关键词: RANTES  呼吸道合胞病毒  毛细支气管炎  基因多态性
英文关键词: RANTES  respiratory syncytial virus  bronchiolitis  genetic polymorphism.
基金项目:南京市医学重点科技发展资助项目(ZKX0306)
作者单位
文惯宇 南京医科大学附属南京儿童医院呼吸科江苏 南京 210008 
赵德育  
田 曼  
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中文摘要:
      目的:探讨正常T淋巴细胞表达和分泌的活性调节蛋白(regulated on activation,normal T cell expressed and secreted,RANTES)启动子-403G/A基因多态性与呼吸道合胞病毒(RSV)致毛细支气管炎(简称毛支)的相关性。方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测238例RSV毛支患者及288例正常对照者RANTES基因-403G/A多态性,酶联免疫吸附试验(ELISA)法检测血清总IgE浓度。结果: RSV毛支组-403G/A基因型及等位基因频率与对照组比较差异均无统计学意义(P > 0.05),但RSV毛支组-403G/A 3个基因型间个体特应性体质阳性率及特应性家族史阳性率比较有显著差异(P < 0.05),并且携带-403A等位基因个体发生特应性体质的危险性是GG纯合子个体的2.57倍(P < 0.05)。RSV毛支组-403G/A基因型间血清IgE水平差异有显著性(P < 0.05)。结论:中国汉族人群RANTES启动子-403G/A基因多态性可能与RSV毛支易感性无关联,但-403A等位基因可能为特应性体质的易感因素。
英文摘要:
      Objective:To explore the genetic association between the regulated on activation, normal T cell expressed and secreted(RANTES) gene promoter -403G/A polymorphism and respiratory syncytial virus(RSV) bronchiolitis in Chinese Han nationality population. Methods:Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was used to identify the polymorphism at position -403 of the RANTES promoter in 238 patients with RSV bronchiolitis and 288 health controls. The total IgE concentrations in serum samples were measured by enzyme-linked immunosorbent assay(ELISA). Results:Compared to normal groups, no significant difference was demonstrated for genotype and allele frequency of the RANTES -403G/A polymorphism in RSV bronchiolitis. However,both the percentage of the personal atopy history and the familial atopy history showed significant difference among the three genetypes of -403G/A in RSV bronchiolitis group(P < 0.05). Interestingly,compared with those carring the GG genotype,subjects with -403A allele demonstrated a 2.57-fold increased risk of personal atopy history. The IgE level indicated significant difference among the three genetypes in RSV bronchiolitis group. Conclusion:The RANTES promoter -403G/A polymorphism may not be associated with the susceptibility of RSV bronchiolitis in Chinese Han nationality population. However,the -403A allele may be a predisposing factor of the personal atopy history.
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