文章摘要
白小明,娄可心,刘宁波,冷 静.选择性COX-2抑制剂celecoxib对肝细胞癌细胞周期的影响[J].南京医科大学学报,2007,(6):534~537
选择性COX-2抑制剂celecoxib对肝细胞癌细胞周期的影响
Induction of cell cycle arrest in hepatocellular carcinoma cells by selective COX-2 inhibitor celecoxib
投稿时间:2006-09-17  
DOI:10.7655
中文关键词: celecoxib  肝细胞癌  细胞周期
英文关键词: celecoxib  hepatocellular carcinoma cell  cell cycle
基金项目:南京医科大学校基金资助项目(NY0510),江苏省高校自然科学基金资助项目(05KJB310081)
作者单位
白小明 南京医科大学病理学系江苏 南京 210029 
娄可心  
刘宁波  
冷 静  
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中文摘要:
      目的:探讨选择性COX-2抑制剂celecoxib对培养的人肝细胞癌细胞的细胞周期的阻滞作用。方法:采用3种常规培养的人肝细胞癌细胞株HUH-7、Hep3B和HepG2,分别给予不同浓度的celecoxib(0、10、25、50 μmol/L),作用不同的时间(0、12、24、48 h),用WST-8法检测细胞的活性,用流式细胞仪检测肝癌细胞的细胞周期变化。结果:WST-8检测发现,随着celecoxib的作用浓度的增加和作用时间的延长,3种肝癌细胞的细胞活性明显下降。流式细胞仪检测发现,3种肝癌细胞在celecoxib 25 μmol/L作用24 h后出现G0/G1期阻滞,HUH-7细胞也出现较弱G2/M期阻滞的现象,48 h时更为明显。结论:选择性COX-2抑制剂celecoxib可以通过明显的细胞周期阻滞作用降低肝细胞癌细胞活性,阻滞的时期主要位于G0/G1期和G2/M期。
英文摘要:
      Objective:To investigate the effect of selective COX-2 inhibitor celecoxib on cell cycle arrest in Hepatocellular carcinoma cells. Methods:Three different human hepatoma cell lines HUH-7, Hep3B and HepG2, were used in this experiment. The cells were treated with various degrees of celecoxib for different time. The cell viability was detected by WST-8. The cell cycle of the hepatoma cells was analyzed by flow cytometry. Results:Celecoxib induced a significant decrease in cell viability in these three hepatoma cell lines. Flow cytometry showed a cell cycle arrest in G0/G1, and a weak arrest in G2/M checkpoint, when the cells were exposed to celecoxib 25 μmol/L for 24 h. And this effect seems to be stronger in 48 h. Conclusion:Celecoxib induce G0/G1 and G2/M cell cycle arrest in human hepatocellular cells, which may play an important role in hepatoma cell growth inhibition.
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