文章摘要
俞静,刘娟,孙岩,刘云,丁国宪.游离脂肪酸对脂肪细胞分化的影响[J].南京医科大学学报,2007,(7):661~665
游离脂肪酸对脂肪细胞分化的影响
Effects and mechanisms of free fatty acid on adipocyte differentiation
投稿时间:2006-12-19  
DOI:10.7655
中文关键词: 11?茁-羟化类固醇脱氢酶1  游离脂肪酸  3T3-L1细胞  脂肪细胞分化
英文关键词: 11?茁-HSD1  free fatty acid  3T3-L1 cells  adipocyte differentiation
基金项目:江苏省135医学重点人才基金资助(30570878)
作者单位
俞静 南京医科大学第一附属医院老年医学科, 江苏 南京 210029 
刘娟  
孙岩  
刘云  
丁国宪  
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中文摘要:
      目的:探讨游离脂肪酸(free fatty acid, FFA)对3T3-L1前脂肪细胞分化的影响及其可能的作用机制?方法:用FFA(100 μg/ml)刺激小鼠成纤维细胞株3T3-L1;构建11?茁-羟化类固醇脱氢酶1(11?茁-hydroxysteroid dehydrogenase type 1, 11?茁-HSD1)的SiRNA 表达质粒pGCsilencerTM H1/TetO1-11?茁-HSD1转染到3T3-L1细胞中并稳定表达,Western blot验证转染效率;采用荧光定量PCR检测脂肪细胞分化相关标志基因:过氧化物酶体增殖物激活受体γ(Peroxisome proliferator-activated receptorγ,PPARγ),CAATT增强子结合蛋白α(CAATT enhancer binding proteins α,C/EBPα),脂蛋白脂酶(Lipoprotein lipase,LPL)和脂肪酸合成酶(Fatty acid synthetase,FAS) mRNA表达量的改变以及FFA刺激时11?茁-HSD1的基因表达水平?结果:FFA刺激后,分化指标PPARγ?C/EBPα?LPL和FAS的mRNA表达量增加,促进了脂肪细胞的分化(P < 0.01); 同时11β-HSD1基因的表达增加(P < 0.05);11β-HSD1基因沉默后,分化指标的mRNA表达量降低,脂肪细胞的分化能力下降(P < 0.01)?结论:FFA和11?茁-HSD1都能促进脂肪细胞分化,FFA的成脂作用可能通过增加11?茁-HSD1的表达实现?
英文摘要:
      Objective:To investigate the effects and mechanisms of free fatty acid(FFA) on 3T3-L1 preadipocyte differentiation. Methods:11?茁-HSD1-SiRNA expression plasmid(pGCsilencerTM H1/TetO1-11?茁-HSD1) was constructed and transfected into 3T3-L1 cells. 100 μg/ml FFA was added to the 3T3-L1 cells. Levels of adipogenesis-associated markers such as Peroxisome proliferator-activated receptorγ(PPARγ), CCAAT enhancer binding protein α(C/EBPα), lipoprotein lipase(LPL), and fatty acid synthetase(FAS) were measured. Results:When the cells treated with FFA, mRNA expressions of PPARγ,C/EBPα,LPL and FAS were up-regulated and the expression of 11?茁-HSD1 also increased. When 11?茁-HSD1 was silenced, the levels of the markers were down-regulated. Conclusion:FFA and 11?茁-HSD1 could promote adipocyte differentiation, and the effect of FFA on adipogenesis carried out through increasing the expression of 11?茁-HSD1.
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