文章摘要
刘洁,殷凯生,卞涛,薛一峰,胡志刚.川芎嗪对哮喘小鼠干细胞因子的抑制作用[J].南京医科大学学报,2007,(7):729~732
川芎嗪对哮喘小鼠干细胞因子的抑制作用
Effect of ligustrazine inhibition on the expression of stem cell factor(SCF) in mouse asth-matic models
投稿时间:2007-01-08  
DOI:10.7655
中文关键词: 川芎嗪  哮喘  干细胞因子
英文关键词: ligustrazine  asthma  stem cells factor
基金项目:
作者单位
刘洁 南京医科大学第一附属医院呼吸科,江苏 南京 210029 
殷凯生 南京医科大学第一附属医院呼吸科,江苏 南京 210029 
卞涛 无锡市第五人民医院呼吸科,江苏 无锡 214073 
薛一峰 无锡市第五人民医院呼吸科,江苏 无锡 214073 
胡志刚 南京医科大学附属无锡一院检验科,江苏 无锡 214002 
摘要点击次数: 1754
全文下载次数: 89
中文摘要:
      目的:探讨川芎嗪对哮喘小鼠血清干细胞因子(stem cell factor,SCF)表达的影响,及抑制气道炎症的作用?方法:24只雄性BALB/c小鼠按随机数字表法分成正常对照组(A组)?哮喘模型组(B组)?小剂量川芎嗪组(C组,40 mg/kg)?大剂量川芎嗪组(D组,80 mg/kg),每组6只?用苏木精-伊红染色观察气道病理改变;用ELISA法检测小鼠血清中SCF含量?结果:小鼠血清中SCF含量哮喘组(114.9 ± 27.3)pmol/L明显高于正常对照组(48.6 ± 11.2)pmol/L(P < 0.01);小剂量川芎嗪组(70.6 ± 7.9)pmol/L与大剂量川芎嗪组(51.4 ± 8.1)pmol/L 均明显低于哮喘组(P均 < 0.01);肺组织中嗜酸性粒细胞(EOS)和淋巴细胞总数,管壁面积/支气管管腔内周长(WA/Pi)和支气管平滑肌面积/支气管管腔内周长(ASM/Pi)哮喘组分别为(32.6 ± 4.5)个/mm2?(1.196 ± 0.111)mm?(0.292 ± 0.027)mm,正常对照组分别为(6.6 ± 1.2)个/mm2?(0.571 ± 0.057)mm?(0.139 ± 0.014)mm,两组比较差异有统计学意义(P均 < 0.01);小剂量川芎嗪组分别为(26.0 ± 2.5)个/mm2?(0.949 ± 0.105)mm?(0.243 ± 0.027)mm,与哮喘组比较差异有统计学意义(P均 < 0.05);大剂量川芎嗪组分别为(12.1 ± 1.6)个/mm2?(0.875 ± 0.111)mm?(0.178 ± 0.023)mm,与哮喘组比较差异有统计学意义(P均 < 0.05)? 结论:川芎嗪可能通过抑制血清SCF表达抑制哮喘小鼠气道炎症?
英文摘要:
      Objective:To identify the ligustrazine effect on asthmatic mice serum stem cell factor(SCF) expression and its effects on inhibiting bronchi inflammation. Methods:Twenty-four male BALB/c mice were randomly divided into four groups:the normal control group(A), the asthmatic model group(B),the low-dose ligustrazine group(C,40 mg/kg) and large-dose ligustrazine group(D,80 mg/kg). There were 6 mice in each group. The bronchi pathological changes were detected by HE dyeing and the SCF content was examined by ELISA. Results:The content of SCF in mice in asthmatic model group[(114.9 ± 27.3)pmol/L] is higher than that in normal control group[(48.6 ± 11.2)pmol/L](P < 0.01). The SCF contents in low-dose ligustrazine group[(70.6 ± 7.9)pmol/L] and in large-dose ligustrazine group[(51.4 ± 8.1)pmol/L] are both lower than that in the asthmatic model group(P < 0.01). In the asthmatic group,the numbers of eosinophils and lymphocytes,the thicknesses of WA/Pi and ASM/Pi were(32.6 ± 4.5)/mm2,(1.196 ± 0.111)mm,(0.292 ± 0.027)mm,respectively;and those of the normal group were(6.6 ± 1.2)/mm2,(0.571 ± 0.057)mm,(0.139 ± 0.014)mm,respectively;there were significant difference between the two groups(all P < 0.01), The numbers of eosinophils and lymphocytes,the thicknesses of WA/Pi and ASM/Pi of the low-dose ligustrazine group were(26.0 ± 2.5)/mm2,(0.949 ± 0.105)mm,(0.243 ± 0.027)mm, respectively. They were lower than those in the asthmatic group(all P < 0.05). The numbers of eosinophils and lymphocytes,the thicknesses of WA/Pi and ASM/Pi of the large-dose ligustrazine group were(12.1 ± 1.6)/mm2,(0.875 ± 0.111)mm,(0.178 ± 0.023)mm,respectively. They were significantly lower than those in the asthmatic group(all P < 0.01). Conclusion:Ligustrazine may control the mice bronchi inflammation by inhibiting the SCF expression.
查看全文   查看/发表评论  下载PDF阅读器