文章摘要
卓莉莉,龚 杰,周 蕾,盛燕辉,杨 荣,孔祥清,曹克将.P19细胞向心肌细胞分化过程中ZNF207基因的表达变化[J].南京医科大学学报,2007,(8):781~784
P19细胞向心肌细胞分化过程中ZNF207基因的表达变化
Changes of the expression of zinc finger protein 207 gene during the differentiation of P19 cells to cardiac myocytes
投稿时间:2007-01-23  
DOI:10.7655
中文关键词: ZNF207基因  P19细胞  细胞分化
英文关键词: Zinc finger protein 207 gene  P19 cells  Cell differentiation
基金项目:国家自然科学基金资助项目(30470709)和江苏省自然科学基金资助项目(BK2004156)
作者单位
卓莉莉 南京医科大学第一附属医院心脏科,江苏 南京 210029 
龚 杰  
周 蕾  
盛燕辉  
杨 荣  
孔祥清  
曹克将  
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中文摘要:
      目的:观察锌指蛋白207基因(ZNF207)在P19细胞向心肌细胞诱导分化过程中表达水平的变化,探讨ZNF207基因与心肌细胞分化之间可能的关系?方法:体外培养P19细胞,应用0.9%二甲基亚砜(DMSO)诱导其向心肌细胞分化,采用RT-PCR技术在细胞分化成熟的不同时段检测P19细胞中ZNF207基因mRNA 表达水平?结果:ZNF207基因在P19细胞向心肌细胞分化的过程中均有表达,随细胞分化成熟,该基因表达水平逐渐升高?经统计学分析发现,在分化第0~2 天该基因表达无差异;第2~10天表达持续性增高,各时间点之间均有差异,差异均有统计学意义(P < 0.05);第10~17天该基因稳定高表达,各时间点之间无差异?结论:ZNF207基因在P19细胞向心肌细胞分化过程中表达逐渐上调,可能有利于心肌细胞的分化和发育?
英文摘要:
      Objective:To investigate the changes of zinc finger protein 207(ZNF207)gene mRNA during the differentiation of P19 cells to cardiac myocytes, and to explore the relationship between ZNF207 gene level and the differentiation stage of cardiac myocytes. Methods:P19 cells were cultured and induced by 0.9%DMSO to differentiate to cardiac myocytes in vitro. Total RNA were extracted from P19 cells during the process of differentiation at various time points:and the levels of ZNF207 mRNA were evaluated by RT-PCR. Results:When P19 cells were induced by DMSO to differentiate to cardiac myocytes, the expression level of ZNF207 gene mRNA was up-regulated and reached the highest level at 10 th day. There was a significant difference between day 2 to day 10(P < 0.05). Conclusion:The expression of ZNF207 gene was up-regulated in the process of the differentiation of P19 cells to cardiac myocytes,which might be involved in the differentiation of cardiac myocytes.
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