文章摘要
王亚梅,马向华,赵 凌,沈 捷.胰岛素对乳腺癌细胞生长的细胞动力学和细胞形态学研究[J].南京医科大学学报,2007,(8):808~811
胰岛素对乳腺癌细胞生长的细胞动力学和细胞形态学研究
Studies on the growth effect and morphology of breast cancer cells stimulated by insulin
投稿时间:2007-01-03  
DOI:10.7655
中文关键词: 胰岛素  胰岛素抵抗  肥胖  乳腺癌细胞
英文关键词: insulin  insulin resistance  obesity  breast cancer cell
基金项目:江苏省教育基金资助项目(05KJD310130)
作者单位
王亚梅 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
马向华 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
赵 凌 南京医科大学第一附属医院中心实验室,江苏 南京 210029 
沈 捷 南京医科大学第一附属医院中心实验室,江苏 南京 210029 
摘要点击次数: 2016
全文下载次数: 200
中文摘要:
      目的:研究胰岛素对乳腺癌细胞株MCF-7细胞生长的作用?方法:分别使用0?25?50?100?200 nmol/L胰岛素作用于细胞株MCF-7细胞,在24?48?72 h采用形态学观察以及MTT比色方法,观察不同浓度胰岛素对MCF-7细胞生长刺激的剂量?时间效应?结果:200 nmol/L胰岛素作用72 h后,MCF-7细胞形态学发生改变,胞质内微腔绒毛减少,线粒体明显扩张?各浓度胰岛素均可明显促进MCF-7细胞增殖,其中,50?100?200 nmol/L胰岛素作用24?48?72 h后差异均有统计学意义(P < 0.05或P < 0.01)?胰岛素对MCF-7细胞的增殖作用呈现一定的时间和剂量依赖效应?结论:胰岛素对乳腺癌细胞株MCF-7细胞增殖有刺激作用,且这种作用呈现一定的剂量?时间依赖效应,结果提示该种细胞因子是促进乳腺癌发生发展的因素之一?
英文摘要:
      Objective:To investigate the growth effect of breast cancer cells by insulin. Methods:MCF-7 cells were exposed to the insulin at various concentrations(25,50,100,200 nmol/L) for 24, 48 and 72 h. The effect of insulin on cell proliferation was assessed by morphologic observing and colorimetric MTT methods. Results:When exposed to the insulin at 200 nmol/L for 72 h, the modality of MCF-7 cells had a significant change. The villus in micro lacuna of cytoplast decreased and mitochondrion expanded obviously. Insulin at different concentrations can significantly promote the cell proliferation, especially at the concentrations of 50, 100 and 200 nmol/L insulin for times of 24,48 and 72 h(P < 0.05 or P < 0.01). And it was showed that this effect was dose- and time- dependent. Conclusion:Insulin can stimulate the proliferation of breast cancer cells in a dose-and time-dependent way. It was indicated that the insulin was a factor to stimulate the proliferation of MCF-7 cells line.
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