胰岛素对乳腺癌细胞生长的细胞动力学和细胞形态学研究
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

江苏省教育基金资助项目(05KJD310130)


Studies on the growth effect and morphology of breast cancer cells stimulated by insulin
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:研究胰岛素对乳腺癌细胞株MCF-7细胞生长的作用-方法:分别使用0-25-50-100-200 nmol/L胰岛素作用于细胞株MCF-7细胞,在24-48-72 h采用形态学观察以及MTT比色方法,观察不同浓度胰岛素对MCF-7细胞生长刺激的剂量-时间效应-结果:200 nmol/L胰岛素作用72 h后,MCF-7细胞形态学发生改变,胞质内微腔绒毛减少,线粒体明显扩张-各浓度胰岛素均可明显促进MCF-7细胞增殖,其中,50-100-200 nmol/L胰岛素作用24-48-72 h后差异均有统计学意义(P < 0.05或P < 0.01)-胰岛素对MCF-7细胞的增殖作用呈现一定的时间和剂量依赖效应-结论:胰岛素对乳腺癌细胞株MCF-7细胞增殖有刺激作用,且这种作用呈现一定的剂量-时间依赖效应,结果提示该种细胞因子是促进乳腺癌发生发展的因素之一-

    Abstract:

    Objective:To investigate the growth effect of breast cancer cells by insulin. Methods:MCF-7 cells were exposed to the insulin at various concentrations(25,50,100,200 nmol/L) for 24, 48 and 72 h. The effect of insulin on cell proliferation was assessed by morphologic observing and colorimetric MTT methods. Results:When exposed to the insulin at 200 nmol/L for 72 h, the modality of MCF-7 cells had a significant change. The villus in micro lacuna of cytoplast decreased and mitochondrion expanded obviously. Insulin at different concentrations can significantly promote the cell proliferation, especially at the concentrations of 50, 100 and 200 nmol/L insulin for times of 24,48 and 72 h(P < 0.05 or P < 0.01). And it was showed that this effect was dose- and time- dependent. Conclusion:Insulin can stimulate the proliferation of breast cancer cells in a dose-and time-dependent way. It was indicated that the insulin was a factor to stimulate the proliferation of MCF-7 cells line.

    参考文献
    相似文献
    引证文献
引用本文

王亚梅,马向华,赵 凌,沈 捷.胰岛素对乳腺癌细胞生长的细胞动力学和细胞形态学研究[J].南京医科大学学报(自然科学版),2007,(8):808-811

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2007-01-03
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期: