文章摘要
许红霞,周建农,莫伏根,侯 宁,胡赛男,束永前.抑癌基因WWOX 和FHIT在结直肠癌中的表达及其临床意义[J].南京医科大学学报,2007,(8):812
抑癌基因WWOX 和FHIT在结直肠癌中的表达及其临床意义
The expression and clinical significance of WWOX and FHIT in colorectal cancer
投稿时间:2006-12-31  
DOI:10.7655
中文关键词: 结直肠癌  包含WW域的氧化还原酶  脆性组氨酸三联体  免疫组织化学  常见脆性位点
英文关键词: sporadic colorectal cancer  WWOX  FHIT  immunohistochemistry  common fragile sites
基金项目:江苏省卫生厅基金重点资助项目(H2009)
作者单位
许红霞 南京医科大学附属江苏省肿瘤医院普外科,江苏 南京 210009 
周建农 南京医科大学附属江苏省肿瘤医院普外科,江苏 南京 210009 
莫伏根 南京医科大学附属江苏省肿瘤医院普外科,江苏 南京 210009 
侯 宁 南京医科大学附属江苏省肿瘤医院普外科,江苏 南京 210009 
胡赛男 南京医科大学附属江苏省肿瘤医院普外科,江苏 南京 210009 
束永前 南京医科大学第一附属医院肿瘤科,江苏 南京 210029 
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中文摘要:
      目的:观察抑癌基因-包含WW域的氧化还原酶(WWOX)和脆性组氨酸三联体(FHIT)在结直肠癌和癌旁正常大肠组织中的表达水平及与临床病理特征?预后之间的关系并探讨两者表达是否具有相关性?方法:应用免疫组织化学PV-9000通用型二步法检测75例结直肠癌和30例癌旁正常大肠组织中WWOX和FHIT基因的蛋白表达?结果:在结直肠癌组织中WWOX和FHIT蛋白阳性表达率明显低于癌旁正常大肠组织(P < 0.05),且这种表达状态与组织学分级?Dukes分期及5年生存率密切相关(P < 0.05),而与部位和年龄无显著相关?WWOX和FHIT蛋白表达之间有相关性,这种相关性可能与常见脆性位点对DNA损伤的易感性相一致有关?结论:WWOX和FHIT基因在结直肠癌的发生?发展过程中可能具有较为重要的作用,并可能联合预测结直肠癌的预后?
英文摘要:
      Objective:To explore the expression and significance of WWOX and FHIT in colorectal cancer. Methods:The expression of WWOX and FHIT was evaluated by immunohistochemical staining(two-step method) in 75 colorectal carcinoma specimens and 30 normal paraneoplastic colorectal tissues,and their correlation with clinicopathological features was analyzed. Results:The rate of WWOX and FHIT positive expression in tumors was 52% and 54.7%,respectively,which was significantly different from that in normal paraneoplastic colorectal tissues as 93.3% and 97.8%. WWOX and FHIT expression were correlated strongly(P < 0.05). The expression of WWOX and FHIT was closely associated with histological grade,Duke stage and 5-year survival rate. And the expression of wwox and FHIT had no significant correlation with neoplastic site and ages. There was a correlation between WWOX expression and FHIT This correlation was consistent with the susceptibility of common fragile sites to DNA damage. Conclusion:The positive expression of WWOX and FHIT was lower than the normal tissues and closely associated with histological grade,Duke stage,and 5-year survival rate(P < 0.05). WWOX and FHIT expression were correlated strongly(P < 0.05). It is suggested that WWOX and FHIT genes may play an important role in the initiation and development of colorectal cancer. They can become targets of predicting the progression of colorectal cancer.
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