文章摘要
周立娜,刘倩琦,刘 峰,寇 敏,朱 敏 .过氧化氢诱导胰岛β细胞凋亡及TIMP-1的保护作用[J].南京医科大学学报,2007,(10):1106~1110
过氧化氢诱导胰岛β细胞凋亡及TIMP-1的保护作用
Protective effects of Tissue inhibitor of metalloproteinase-1 on apoptosis of insulin-producing RINm5F cells induced by hydrogen peroxide
投稿时间:2007-03-24  
DOI:10.7655
中文关键词: 胰岛β细胞  凋亡  基质金属蛋白酶抑制剂-1  过氧化氢
英文关键词: β cell  apoptosis  tissue inhibitor of metalloproteinase-1  H2O2
基金项目:
作者单位
周立娜 南京医科大学附属南京儿童医院,江苏 南京 210008 
刘倩琦 南京医科大学儿科医学研究所,江苏 南京 210029 
刘 峰 南京医科大学儿科医学研究所,江苏 南京 210029 
寇 敏 南京医科大学附属南京儿童医院,江苏 南京 210008 
朱 敏 南京医科大学附属南京儿童医院,江苏 南京 210008 
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中文摘要:
      目的:研究过氧化氢诱导的胰岛β细胞凋亡及基质金属蛋白酶抑制剂-1(TIMP-1)的保护作用和可能机制?方法:过氧化氢诱导大鼠胰岛瘤细胞RINm5F细胞凋亡,实验组予以TIMP-1干预?应用MTT法和Caspase-3活性检测来比较各组间细胞活力和Caspase-3活性的差异,检测超氧化物歧化酶(SOD)活性来反映各组之间抗氧化能力的差异?结果:应用0.1 mmol/L过氧化氢干预1 h,能明显诱导RINm5F细胞凋亡,过氧化氢刺激前TIMP-1(50 μg/L)预处理1 h较单独过氧化氢刺激组细胞活力上升约35%,Caspase-3活性下降约21%,SOD活性增加约46%,两组比较差异有显著性意义(P < 0.05)?结论:TIMP-1对过氧化氢诱导的RINm5F细胞的凋亡有保护作用,其机制可能是通过诱导SOD酶的活性增加来实现的?
英文摘要:
       Objective:To investigate the effect of tissue inhibitor of metalloproteinase-1(TIMP-1) on the apoptosis of β cell induced by hydrogen peroxide and the mechanism of TIMP-1 action. Methods:The insulin-producing rat cell line RINm5F cells were cultured and stimulated by H2O2 with or without the addition of TIMP-1(50 μg/L). Cell viability,Caspase-3 and superoxide dismutase(SOD) activity were determined after the cells were exposed to H2O2 and TIMP-1. Results:Cell viability was decreased obviously in RINm5F cells after incubation with H2O2(0.1 mmol/L) for 1 hour alone. In the cells after treatment with TIMP-1(50 μg/L) for 1 hour prior to H2O2,cell viability and SOD activity were remarkably increased and the activity of Caspase-3 was lower significantly than that in the cells treated by H2O2 alone(P < 0.05). Conclusion:TIMP-1 can protect RINm5F cells from apoptosis induced by hydrogen peroxide,which possibly involves in raising the activity of SOD.
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