The effects of necrosis factor alpha and interleukin 6 on liver regeneration following partial liver transplantation in rats
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摘要:
目的：研究肝再生相关细胞因子肿瘤坏死因子（TNF-α）和白介素-6（IL-6）在大鼠移植肝内的表达及其与肝脏再生的关系。方法：建立稳定的冷缺血45 min和10 h 50%大鼠部分肝移植模型，50%肝切除为对照组。术后免疫组织化学检测各组增殖细胞核抗原（PCNA）、TNF-α、IL-6的表达，RT-PCR检测术后2 h TNF-α、IL-6 mRNA的表达，并探讨TNF-α和IL-6的变化规律及其对大鼠部分肝移植术后肝再生的影响。结果：冷缺血10 h肝移植组PCNA表达明显下降。与对照组相比，冷缺血45 min组大鼠移植肝内细胞因子TNF-α、IL-6表达明显增加，高于对照组和冷缺血10 h组（P < 0.05），持续的时间也延长到移植后24 h以上。结论：与大鼠肝切除后诱导肝再生的机制相似，TNF-α、IL-6等细胞因子在移植后肝脏再生的早期启动过程中非常重要。短暂冷缺血促进大鼠肝移植后的肝脏再生，较长时间冷缺血降低部分肝移植术后肝再生。
Abstract:
Objective：To study the effects of necrosis factor alpha（TNF-α） and interleukin 6（IL-6）on liver regeneration in grafts using a novel model of partial liver transplantation in rats. Methods：Rats subjected to 50% liver resection（control） were compared with rats that underwent total hepatectomy and 50% partial liver transplantation. Livers were preserved at 4℃ for 45 min and 10 h respectively. The expression of the proliferative cell nuclear antigen（PCNA），TNF-α and IL-6 of graft in rats were evaluated by immuno histochemical method 1，2，4 d after operation，respectively. The mRNA of TNF-α and IL-6 was detected by reversed transcript polymerase chain reaction（RT-PCR）. And the relationship of graft regeneration and those cytokines were investigated.Results：Prolonged time of cold ischemia（10 h） was associated with a dramatic decrease of PCNA（P < 0.05）. TNF-α and IL-6 was significantly decreased in recipient rats compared with liver resection and 45 minutes of cold preservation group（P < 0.05）. The peak of liver regeneration delayed 24 h postoperation compared with liver resection group. Conclusion：TNF-α and IL-6 are significantly associated with the regenerative activation of the partial liver transplantation in early stage. Prolonged cold ischemia decreases the liver regeneration by expression of TNF-α and IL-6 production in graft.
