文章摘要
成 峰,王学浩,华志元,黄 蓬.TNF-α和IL-6在大鼠部分肝移植术后肝再生的作用[J].南京医科大学学报,2007,(11):1267~1270
TNF-α和IL-6在大鼠部分肝移植术后肝再生的作用
The effects of necrosis factor alpha and interleukin 6 on liver regeneration following partial liver transplantation in rats
投稿时间:2007-06-22  
DOI:10.7655
中文关键词: 冷缺血  部分肝移植  白介素- 6  肿瘤坏死因子-α  肝脏再生  大鼠
英文关键词: cold ischemia  partial liver transplantation  interleukin-6  tumor necrosis factor alpha  liver regeneration
基金项目:江苏省卫生厅医学重点课题(K200414)
作者单位
成 峰 南京医科大学第一附属医院肝移植中心江苏 南京 210029 
王学浩  
华志元  
黄 蓬  
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中文摘要:
      目的:研究肝再生相关细胞因子肿瘤坏死因子(TNF-α)和白介素-6(IL-6)在大鼠移植肝内的表达及其与肝脏再生的关系。方法:建立稳定的冷缺血45 min和10 h 50%大鼠部分肝移植模型,50%肝切除为对照组。术后免疫组织化学检测各组增殖细胞核抗原(PCNA)、TNF-α、IL-6的表达,RT-PCR检测术后2 h TNF-α、IL-6 mRNA的表达,并探讨TNF-α和IL-6的变化规律及其对大鼠部分肝移植术后肝再生的影响。结果:冷缺血10 h肝移植组PCNA表达明显下降。与对照组相比,冷缺血45 min组大鼠移植肝内细胞因子TNF-α、IL-6表达明显增加,高于对照组和冷缺血10 h组(P < 0.05),持续的时间也延长到移植后24 h以上。结论:与大鼠肝切除后诱导肝再生的机制相似,TNF-α、IL-6等细胞因子在移植后肝脏再生的早期启动过程中非常重要。短暂冷缺血促进大鼠肝移植后的肝脏再生,较长时间冷缺血降低部分肝移植术后肝再生。
英文摘要:
       Objective:To study the effects of necrosis factor alpha(TNF-α) and interleukin 6(IL-6)on liver regeneration in grafts using a novel model of partial liver transplantation in rats. Methods:Rats subjected to 50% liver resection(control) were compared with rats that underwent total hepatectomy and 50% partial liver transplantation. Livers were preserved at 4℃ for 45 min and 10 h respectively. The expression of the proliferative cell nuclear antigen(PCNA),TNF-α and IL-6 of graft in rats were evaluated by immuno histochemical method 1,2,4 d after operation,respectively. The mRNA of TNF-α and IL-6 was detected by reversed transcript polymerase chain reaction(RT-PCR). And the relationship of graft regeneration and those cytokines were investigated.Results:Prolonged time of cold ischemia(10 h) was associated with a dramatic decrease of PCNA(P < 0.05). TNF-α and IL-6 was significantly decreased in recipient rats compared with liver resection and 45 minutes of cold preservation group(P < 0.05). The peak of liver regeneration delayed 24 h postoperation compared with liver resection group. Conclusion:TNF-α and IL-6 are significantly associated with the regenerative activation of the partial liver transplantation in early stage. Prolonged cold ischemia decreases the liver regeneration by expression of TNF-α and IL-6 production in graft.
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