文章摘要
朱春富,徐 皓,陈小平,戚美芬,纪俊标,高 翔,孙 立.淤血预处理对大鼠小肠淤血再灌注损伤的保护作用[J].南京医科大学学报,2008,28(4):513~517
淤血预处理对大鼠小肠淤血再灌注损伤的保护作用
Effects of congestion preconditioning on acute intestinal venous congestion reperfusion injury in rats
投稿时间:2007-11-30  
DOI:10.7655
中文关键词: 小肠  淤血再灌注  损伤  预处理
英文关键词: intestine  congestion and reperfusion  injury  congestion preconditioning
基金项目:南京医科大学科技发展基金资助(NY04053)
作者单位
朱春富 南京医科大学附属常州二院普外科江苏 常州 213003 
徐 皓 南京医科大学第一附属医院普外科江苏 南京 210029 
陈小平 南京医科大学附属常州二院普外科江苏 常州 213003 
戚美芬 南京医科大学附属常州二院普外科江苏 常州 213003 
纪俊标 南京医科大学第一附属医院普外科江苏 南京 210029 
高 翔 南京医科大学附属常州二院普外科江苏 常州 213003 
孙 立 南京医科大学附属常州二院普外科江苏 常州 213003 
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中文摘要:
      目的:研究淤血预处理对大鼠小肠淤血再灌注损伤的保护作用。方法:30只大鼠随机分为3组:淤血再灌注组、淤血预处理组和假手术组。通过阻断门静脉45 min、再灌注60 min制作小肠淤血再灌注模型。淤血预处理组在门静脉夹闭前先短暂夹闭门静脉5 min,开放5 min,并重复1次。假手术组不夹闭门静脉。于夹闭门静脉前、开放后15、30、45和60 min分别测定门静脉的血流量,再灌注后60 min取末端回肠组织分别作病理学检查、MPO和免疫组化ICAM-1测定。结果:淤血预处理组肠黏膜损伤较淤血再灌注组明显减轻,且肠组织MPO活性和ICAM-1表达均低于淤血再灌注组,开放血流后门静脉血流量均明显升高。结论:淤血预处理对肠道淤血再灌注损伤具有保护作用。
英文摘要:
      Objective:To investigate the effects of congestion preconditioning on acute intestinal venous congestion reperfusion injury in rats. Methods:A total of 30 SD rats were divided into three groups,the acute intestinal venous congestion reperfusion group(CR group),the congestion preconditioning group(group CP) and the sham operation group(group SO). To induce intestinal venous congestion,portal vein was clamped for 45 min,then unclamped for 60 min. In group CP,portal vein was clamped for 5 min,then unclamped for 5 min with one repeat of this procedure before model construction. In group SO,all the procedures were conducted except for clamping portal vein. Portal flow was measured with electromagnetic flowmeter at different time point of congestion and reperfusion. After reperfusion for 60 min,ileum samples were collected for MPO and ICAM-1 determination as well as pathological biopsy. Results:In group CP,it was found that the intestinal mucosa was less damaged than that of group CR with Chiu’s Grade Classification. Meanwhile,intestinal MPO activity and ICAM-1 expression in group CP were also much lower than those in group CR. There was no significant difference in portal flow before the clamping of portal vein among the 3 groups. After reperfusion,the portal flow in group CP significantly increased in compare with that of group CR. Conclusion:Congestion preconditioning could alleviate the injury of acute intestinal venous congestion reperfusion in rats.
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