文章摘要
陈兰兰,孟 明,万 琪,陈伟贤.辅酶Q10能有效抑制鱼藤酮诱导的细胞毒性作用[J].南京医科大学学报,2008,28(5):646~649
辅酶Q10能有效抑制鱼藤酮诱导的细胞毒性作用
The study of protective effect and the mechanism of coenzyme Q10 on rotenone-induced dopaminergic neurons
投稿时间:2007-12-11  
DOI:10.7655
中文关键词: 多巴胺能神经元  辅酶Q10  鱼藤酮  线粒体膜电位?
英文关键词: dopaminergic neurons  coenzyme Q10  rotenone  mitochondrial membrane.
基金项目:
作者单位
陈兰兰 南京医科大学第一附属医院神经内科,江苏 南京 210029 
孟 明  
万 琪  
陈伟贤  
摘要点击次数: 976
全文下载次数: 96
中文摘要:
      目的:探讨辅酶Q10对鱼藤酮介导的多巴胺能神经元损伤是否具有保护作用并研究其机制?方法:原代培养孕14 d的胎鼠中脑多巴胺能神经元,以不同剂量的鱼藤酮建立细胞凋亡模型?通过TH免疫细胞化学进行鉴定,测定LDH值以了解细胞活力,以罗丹明123染料进行流式细胞仪检测线粒体膜电位(△Ψm)?结果:鱼藤酮干预多巴胺能神经元24 h后,细胞活力及△Ψm均明显下降,与0 nmol/L鱼藤酮组比较,15 nmol/L及30 nmol/L鱼藤酮组的LDH值(U/ml)显著增加(P < 0.05),其△Ψm(%)则明显下降(P < 0.05);辅酶Q10 12 h能显著缓解细胞△Ψm的下降,与15 nmol/L鱼藤酮组比较,50 μmol/L及100 μmol/L辅酶Q10预处理组的△Ψm(%)明显上升(P < 0.05)?结论:辅酶Q10能有效抑制鱼藤酮诱导的细胞毒性作用?
英文摘要:
      Objective:To study the neuroprotective effects and the mechanism of CoQ10 on Rotenone-induced dopaminergic neurons. Methods:Using Rotenone upon dopaminergic mesencephalic neurons in primary culture to set up cell model of Parkinson’s disease (PD) in vitro. The dopaminergic neurons were identified by tyrosine hydroxylase(TH) immunocytochemistry. A lactate dehydrogenase (LDH) assay kit was used to measure the transudatory amount of LDH to reflect the cell viability. By measuring the intracellular Rhodamine 123 fluorescene density,mitochondrial potential(△Ψm) was evaluated. Results:Rotenone can dramatically reduce the cell viability and Ψm(P < 0.05). The rate of cell viability of dopaminergic neurons in group 15 nmol/L and 30 nmol/L rotenone were increased,and the percentage of △Ψm in group with 15 nmol/L and 30 nmol/L rotenone were increased(P < 0.05) as compared with group without rotenone treatment; while pretreatment with 50 μmol/L and 100 μmol/L CoQ10 can significantly prevent 15 nmol/L Rotenone-induced dopaminergic neurons mitochondrial depolarization(P < 0.05). Conclusion:CoQ10 can effectively protect the rotenone-induced dopaminergic neurons.
查看全文   查看/发表评论  下载PDF阅读器
关闭