文章摘要
刘兴波,雷晓婷,刘 源,徐冬晨,王 彤,丁新生.大鼠脊髓损伤后神经生长因子BDNF\轴突导向因子slit-2的表达变化[J].南京医科大学学报,2008,28(7):868~871
大鼠脊髓损伤后神经生长因子BDNF\轴突导向因子slit-2的表达变化
Expression changes of BDNF\slit-2 during the spinal cord injury of rats
投稿时间:2008-01-21  
DOI:10.7655
中文关键词: 脊髓损伤  神经生长因子BDNF  轴突导向因子slit-2  免疫组化
英文关键词: spinal cord injury(SCI)  nerve growth cue BDNF  axon guidance cue slit-2  immunohistochemistry
基金项目:国家自然科学基金资助项目(NO30671018)
作者单位
刘兴波 南京医科大学第一附属医院神经内科,江苏 南京 210029 
雷晓婷 南京医科大学第一附属医院神经内科,江苏 南京 210029 
刘 源 南京医科大学第一附属医院神经内科,江苏 南京 210029 
徐冬晨 南京医科大学第一附属医院康复科,江苏 南京 210029 
王 彤 南京医科大学第一附属医院康复科,江苏 南京 210029 
丁新生 南京医科大学第一附属医院神经内科,江苏 南京 210029 
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中文摘要:
      目的:研究脊髓损伤后神经生长因子BDNF和轴突导向因子slit-2的表达变化,同时观察大鼠行为学?脊髓形态学及体感诱发电位(SEP)的变化特点?方法:雄性SD大鼠45只,随机分成正常组,假手术组和手术后1?3?5?7?14?21?28天组,每组各5只?免疫组化法观察神经生长因子BDNF\slit-2的表达变化,计算阳性细胞灰度值,并进行统计学分析?BBB法进行功能评分?HE染色观察脊髓损伤后不同时期的形态学变化?结果:大鼠脊髓损伤后,肢体瘫痪从第7天开始明显恢复直至28天?免疫组化结果正常组和假手术组脊髓前角BDNF\slit-2的表达较低?脊髓损伤24 h后,损伤位点前角神经元中,BDNF\slit-2表达活跃,3天表达达高峰?形态学显示了损伤后脊髓出血吸收?变性的过程?结论:成年大鼠脊髓损伤后,神经生长因子BDNF和轴突导向因子slit-2随之上调,呈一定规律性,提示两者与脊髓损伤后神经再生相关?且BDNF? slit-2主要表达于脊髓前角,提示与脊髓损伤后肢体运动功能恢复密切相关?
英文摘要:
       Objective:To investigate the expression of BDNF and axon guidanc cue slit-2 after the spinal cord injury,simultaneously observe the ethology, morphology and SEP changes of rats. Methods:Male Sprague-Dawley rats were randomly divided into normal group,the sham-operation group and the operation group(1,3,5,7,14,21,28 days sub groups), each group with 5 rats. The expression of BDNF and slit-2 were detected through immunohistochemistry methods. The positive cells were analyzed by a computer image analysis system. Function changes were evaluated by BBB method, the morphological changes were obsered by HE staining, and the SEP change was also analyzed. Results:The limb function of experimental rats significantly recovered from 7 d to 28 d. The immunohistochemistry results indicated that expression of BDNF\slit-2 in the angulus anterior was low in either normal or sham operation group,while BDNF\slit-2 expressed actively at 24 h in the operation group,reached the peak at 3 days after operation. The morphology gradually changed from local hematoma to tissue degeneration. Conclusion:With the spinal cord injury of adult rats, BDNF and slit-2 increased with regularity, indicating the correlation between the two factors and the neuranagenesis. BDNF and slit-2 were mainly expressed in the ventricolumna,implying they might be correlated with the limb movement function restore.
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