文章摘要
陶春香,苗登顺.甲状旁腺素和活性维生素D在调节肾脏钙结合蛋白中的协同作用[J].南京医科大学学报,2008,28(7):894~898
甲状旁腺素和活性维生素D在调节肾脏钙结合蛋白中的协同作用
Interaction of parathyroid hormone(PTH) and 1,25(OH)2D3 on the regulation of renal calcium binding proteins
投稿时间:2008-03-07  
DOI:10.7655
中文关键词: 甲状旁腺素  1,25(OH)2D3  钙结合蛋白D28K  钙结合蛋白D9K
英文关键词: parathyroid hormone  1,25(OH)2D3  calbindin D28K  calbindin D9K
基金项目:
作者单位
陶春香 南京医科大学骨与干细胞研究中心,江苏 南京 210029 
苗登顺  
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中文摘要:
      目的:明确甲状旁腺素(PTH)和1,25-二羟维生素D3[1,25(OH)2D3]在调节肾脏钙结合蛋白中的各自和协同作用?方法:采用免疫组织化学方法观察PTH基因敲除?1α羟化酶基因敲除及双基因敲除对钙结合蛋白D28K和D9K在小鼠肾脏不同部位表达的影响?结果:钙结合蛋白D28K和D9K均定位于肾脏皮质远曲小管及髓质集合管上皮细胞的胞浆内?两种钙结合蛋白在PTH基因敲除小鼠肾脏的表达均明显减少?它们的减少在1α羟化酶基因敲除小鼠更为明显,而以双基因敲除小鼠最为显著?结论:PTH和活性维生素D均能调节钙结合蛋白在肾脏的表达,而且两者具有协同作用?
英文摘要:
      Objective:To determine the simple effect and interaction of parathyroid hormone(PTH) and 1,25(OH)2D3 on the regulation of renal calcium binding proteins. Methods:The expression of renal calbindin D28K and D9K were examined in mice with targeted knockout of the PTH or 1α(OH)ase or both genes by immunohistochemistry. Results:The calbindin D28K and D9K were localized to intracytoplasm of epithelia in the renal cortical distal tubules and medullary collecting ducts. The expression of calbindin D28K and D9K were reduced significantly in kidney in PTH knockout mice, more dramatically decreased in 1α(OH)ase knockout mice and the most dramatically decreased in the double mutant mice. Conclusion:PTH and 1,25(OH)2D3 could play collaborative roles in modulating the expression of calbindin D28K and D9K in the kidney.
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