文章摘要
张前德,时彦标,谈文峰,王 芳.雷公藤甲素对类风湿关节炎滑膜成纤维细胞系MH7A中VEGF?MMP-9水平变化的影响[J].南京医科大学学报,2008,28(7):902~905
雷公藤甲素对类风湿关节炎滑膜成纤维细胞系MH7A中VEGF?MMP-9水平变化的影响
Effect of triptolide on the changes of VEGF and MMP-9 levels in the rheumatoid arthritis fibroblast-like synoviocyte line,MH7A
投稿时间:2008-01-20  
DOI:10.7655
中文关键词: 雷公藤甲素  类风湿性关节炎  血管内皮生长因子  基质金属蛋白酶-9
英文关键词: Triptolide  rheumatoid arthritis  vascular endothelial growth factor(VEGF)  matrix metallproteinases(MMP-9)
基金项目:
作者单位
张前德 南京医科大学,江苏 南京 210029 
时彦标 南京中医药大学第一临床医学院,江苏 南京 210029 
谈文峰 南京医科大学第一附属医院风湿免疫科,江苏 南京 210029 
王 芳 南京医科大学第一附属医院基因实验室,江苏 南京 210029) 
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中文摘要:
      目的:研究雷公藤甲素对类风湿关节炎(RA)滑膜成纤维细胞系MH7A培养上清液中血管内皮生长因子(VEGF)?基质金属蛋白酶-9(MMP-9)水平变化的影响?方法:肿瘤坏死因子-α(TNF-α)刺激RA滑膜成纤维细胞系MH7A后,以不同剂量TP干预,酶联免疫吸附试验(ELISA)检测细胞培养液上清中VEGF?MMP-9水平的变化?结果:TNF-α刺激后,细胞培养上清液中VEGF及MMP-9分泌不同程度增加?TP各剂量组(1?5?25?75 μg/ml)在不同时间点(24?48?72 h)均可以明显下调培养上清液中MMP-9和VEGF的水平,与地塞米松对照组比有显著差异?结论:雷公藤甲素能抑制血管新生有关细胞因子VEGF?MMP-9的表达?
英文摘要:
      Objective:To study the effect of triptolide on the expression of VEGF and MMP-9 in the rheumatoid arthritis fibroblast-like synoviocyte line,MH7A. Methods:Rheumatoid arthritis fibroblast-like synoviocyte line,MH7A were incubated for 24 hours with 20 ng/ml of recombinant human TNF-α then were treated with Triptolide of different concentration(1 μg/ml,5 μg/ml,25 μg/ml and 75 μg/ml). Levels of vascular endothelial growth factor(VEGF) and matrix metallproteinases(MMP-9)were measured in cell supernatants by Enzyme-Linked ImmunoSorbent Assay(ELISA). Results:After TNFα stimulation,VEGF and MMP-9 production was highly increased in the supernatants. Triptolide caused a dose-dependent decrease in VEGF and MMP-9. The inhibited effect of Triptolide on the expression of VEGF and MMP-9 is stronger than that of Dexamethasone. Conclusion:Triptolide can inhibit the regulative factor of angiogenesis VEGF and MMP-9 expression.
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