雷公藤甲素对类风湿关节炎滑膜成纤维细胞系MH7A中VEGF?MMP-9水平变化的影响
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Effect of triptolide on the changes of VEGF and MMP-9 levels in the rheumatoid arthritis fibroblast-like synoviocyte line,MH7A
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    摘要:

    目的:研究雷公藤甲素对类风湿关节炎(RA)滑膜成纤维细胞系MH7A培养上清液中血管内皮生长因子(VEGF)-基质金属蛋白酶-9(MMP-9)水平变化的影响-方法:肿瘤坏死因子-α(TNF-α)刺激RA滑膜成纤维细胞系MH7A后,以不同剂量TP干预,酶联免疫吸附试验(ELISA)检测细胞培养液上清中VEGF-MMP-9水平的变化-结果:TNF-α刺激后,细胞培养上清液中VEGF及MMP-9分泌不同程度增加-TP各剂量组(1-5-25-75 μg/ml)在不同时间点(24-48-72 h)均可以明显下调培养上清液中MMP-9和VEGF的水平,与地塞米松对照组比有显著差异-结论:雷公藤甲素能抑制血管新生有关细胞因子VEGF-MMP-9的表达-

    Abstract:

    Objective:To study the effect of triptolide on the expression of VEGF and MMP-9 in the rheumatoid arthritis fibroblast-like synoviocyte line,MH7A. Methods:Rheumatoid arthritis fibroblast-like synoviocyte line,MH7A were incubated for 24 hours with 20 ng/ml of recombinant human TNF-α then were treated with Triptolide of different concentration(1 μg/ml,5 μg/ml,25 μg/ml and 75 μg/ml). Levels of vascular endothelial growth factor(VEGF) and matrix metallproteinases(MMP-9)were measured in cell supernatants by Enzyme-Linked ImmunoSorbent Assay(ELISA). Results:After TNFα stimulation,VEGF and MMP-9 production was highly increased in the supernatants. Triptolide caused a dose-dependent decrease in VEGF and MMP-9. The inhibited effect of Triptolide on the expression of VEGF and MMP-9 is stronger than that of Dexamethasone. Conclusion:Triptolide can inhibit the regulative factor of angiogenesis VEGF and MMP-9 expression.

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张前德,时彦标,谈文峰,王 芳.雷公藤甲素对类风湿关节炎滑膜成纤维细胞系MH7A中VEGF?MMP-9水平变化的影响[J].南京医科大学学报(自然科学版),2008,28(7):902-905

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  • 收稿日期:2008-01-20
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