Objective:To establish the method to identify protein using LC-MALDI. Methods:To investigate if the capillary liquid chromatography coupled with matrix-assisted laser desorption/ionization mass spectrometry technology is an available method to proteins sequence by comparing with the most common sequencing method of MALDI-TOF/TOF-MS. Results:17 tryptic peptides can be identified using MALDI-TOF/TOF-MS with 31.5% sequence coverage and 130 score while 35 peptides can be identified using CapLC coupled with MALDI-TOF/TOF-MS method with 59.35% sequence coverage and 2 976 score. Conclusion:The method of proteins sequence using CapLC coupled with MALDI-TOF/TOF-MS is available. Moreover,this identification method is more precise and effective than MALDI-TOF/TOF-MS in protein identification.