文章摘要
万锦平,黄建安,穆传勇,刘 皓,鲁常青.非小细胞肺癌endocan?FLK-1的表达与血管生成相关性研究[J].南京医科大学学报,2009,29(1):28~31
非小细胞肺癌endocan?FLK-1的表达与血管生成相关性研究
The Expression of endocan and FLK-1 and their correlations with angiogenesis in non small cell lung cancer
投稿时间:2008-09-24  
DOI:10.7655
中文关键词: 非小细胞肺癌  内皮细胞特异分子-1  血管内皮细胞生长因子受体  微血管密度
英文关键词: non-small cell lung cancer  endocan  kinase insert domain containing receptor/fetal liver kinase-1  microvessel density
基金项目:
作者单位
万锦平 常州市第一人民医院呼吸科,江苏 常州 213003 
黄建安 常州市第一人民医院呼吸科,江苏 常州 213003 
穆传勇 苏州大学附属第一医院呼吸科, 江苏 苏州 215000 
刘 皓 常州市第一人民医院呼吸科,江苏 常州 213003 
鲁常青 常州市第一人民医院呼吸科,江苏 常州 213003 
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中文摘要:
      目的:研究内皮细胞特异分子-1(endothelial cell specific-1,endocan)?血管内皮细胞生长因子受体(KDR/FLK-1)在非小细胞肺癌(NSCLC)中的表达及其临床意义,同时探讨endocan?FLK-1与非小细胞肺癌血管生成的相关性?方法:应用免疫荧光技术检测60例手术切除的肺癌组织?21例癌旁肺组织(距癌组织5 cm以上)中的endocan表达水平;免疫组化SP法检测FLK-1表达, 并计数微血管密度(MVD)?结果:endocan?FLK-1的表达在NSCLC组织中明显高于癌旁组织(P均 < 0.05);endocan?FLK-1的表达与NSCLC的淋巴结转移?TNM分期正相关(P均 < 0.05),而与NSCLC的组织学分类无关(P均 > 0.05);在NSCLC中MVD?endocan?FLK-1阳性表达组明显高于二者阴性表达组(P均 < 0.05);endocan?FLK-1在NSCLC中的表达呈正相关(r = 0.888 9,P < 0.05)?结论:endocan?FLK-1在NSCLC组织的血管发生?进展等病理过程有重要作用?
英文摘要:
      Objective:To investigate the expression of endocan and FLK-1 in NSCLC, and to explore the correlation with angiogenesis and other clinical pathophysiological characteristics of NSCLC. Methods:The expression of endocan was detected by using immunofluorescence technique in 60 specimens of NSCLC and 21 specimens of adjacent non-cancerous tissues as control group. Immunohistochemical S-P method was performed to estimate the expression of FLK-1 and MVD. Results:The endocan-positive rate was 78.33%(47/60) in NSCLC group and 14.29%(3/21) in control group. The positive rate of FLK-1 was 71.67%(43/60)in NSCLC group and 19.05%(4/21) in control group. There was a significant difference between them(χ2 = 27.01,17.68; all P < 0.05). In stage Ⅰ+Ⅱ and Ⅲ+Ⅳ of NSCLC, the positive rates of endocan were 67.64%(23/34) and 92.30%(24/26) while the positive rates of FLK-1 were 58.82%(20/34) and 88.46%(23/26). The differences of endocan and FLK-1 expression between stageⅠ+Ⅱ and Ⅲ+Ⅳ were significant respectively(χ2=5.28, 6.37, all P < 0.05). In the group with lymph node metastasis and the group without lymph node metastasis, the positive rates of endocan were 84.78%(39/46) and 57.14%(8/14), the positive rates of FLK-1 were 80.43%(37/46) and 42.86%(6/14). The differences of endocan and FLK-1 expression between the two groups were also significant respectively(χ2 = 4.83,7.46,all P < 0.05). The MVDs in the endocan-positive group and the endocan-negative group were 25.16 ± 5.12 and 18.97 ± 3.28 respectively, the difference was significant(t = 4.52, P < 0.05). The MVDs in the FLK-1-positive group and the FLK-1-negative group were 24.65 ± 4.34 and 18.92 ± 3.35 respectively, the difference was also significant(t = 5.23,P < 0.05). There was positive correlation between the expressions of endocan and FLK-1 in NSCLC(r = 0.8889, P < 0.05). However, The positive rates of endocan were 77.42%(24/31) in adenocarcinoma, 80.00%(20/25) in squamous cell carcinoma and 75.00%(3/4) in large cell lung cancer. The positive rates of FLK-1 were 70.96%(22/31) in adenocarcinoma,72.00%(18/25) in squamous cell carcinoma and 75.00%(3/4) in large cell lung cancer. There was no significant difference among them(χ2=3.63,1.79, all P > 0.05). Conclusion:Endocan and FLK-1 might play very important roles in tumor angiogenesis in NSCLC.
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