Objective:To investigate the effect of glucocorticoid on the expression of 11β-hydroxysteroid dehydrogenase in the pig iliac artery endothelial cell(PIEC) under hyperglycemia expression. Methods:PIEC were incubated at a different concentration of glucocorticoid under hyperglycemia(30 mmol/L),the expression of 11β-HSD1,11β-HSD2 were measured by RT-PCR technique, the protain expression of 11β-HSD1,11β-HSD2 were measured by Western-blot technique. Results:Under hyperglycemia, glucocorticoid can upregulate the expression of 11β-HSD1 mRNA and protein(P < 0.05) compared with the control group,and increased with the concertration of cortisone. The expression of 11β-HSD2 mRNA and protein had not change with the concentration of cortisol. Conclusion: High glucose and glucocorticoid can increase the expression of 11β-HSD1 mRNA and protein.