文章摘要
顾海燕,赵德育.维生素D缺乏对大鼠肺发育影响的形态学研究[J].南京医科大学学报,2009,29(1):81~85
维生素D缺乏对大鼠肺发育影响的形态学研究
Effects of vitamin D deficiency on rat lung morphogenesis
投稿时间:2008-08-25  
DOI:10.7655
中文关键词: 维生素D  大鼠  肺发育
英文关键词: vitamin D  rats  lung development
基金项目:
作者单位
顾海燕 南京医科大学附属南京儿童医院呼吸科,江苏 南京 210029 
赵德育  
摘要点击次数: 1599
全文下载次数: 243
中文摘要:
      目的:研究维生素D(VitD)缺乏对大鼠肺发育的形态学影响?方法:雌性SD孕鼠随机分为正常对照组?VitD缺乏模型组及干预组3组,每组6只?对照组予光照及含VitD的正常饲料喂养;模型组及干预组予以避光?不含VitD的饲料喂养,2周后与成熟SD 雄性大鼠交配,于孕17?18?19天干预组以活性VitD(0.5 mg/kg)灌胃,并恢复光照及正常喂养;模型组及对照组予以等体积生理盐水灌胃?各组取孕20天的胎肺及生后1天新生鼠肺组织,通过光镜观察和电镜技术,分析研究VitD缺乏对肺发育的形态学影响?结果:光镜下,孕20天模型组及干预组胎肺肺泡平均表面积?平均呼吸膜周径均小于同龄正常对照组(P < 0.05),平均肺泡间隔厚度大于对照组(P < 0.05);生后1天新生鼠模型组肺泡平均表面积?平均呼吸膜周径小于对照组(P < 0.05),平均肺泡间隔厚度大于对照组(P < 0.05),干预组肺泡平均表面积?平均呼吸膜周径大于模型组,平均间隔厚度小于模型组(P < 0.05)?电镜下,孕20天及生后1天的模型组的板层小体数量均明显少于对照组,且孕20天模型组糖原沉积丰富,生后1天模型组板层小体结构疏松,可见板层小体排空现象;干预组上述变化较模型组有所改善?结论:孕期VitD缺乏抑制了孕晚期胎鼠及新生大鼠的肺发育,补充活性维生素D可逆转上述抑制作用?
英文摘要:
      Objective:To study the effect of vitamin D on rat lung morphogenesis. Methods:Sprague-Dawley pregnant rats were divided into 3 groups randomly including normal control group, vitamin D deficiency experiment model group and intervention group, with 6 rats in each group. We fed the model and intervention groups with fodder without vitamin D away from light, while the control group received normal fodder and normal light for two weeks. Then the female rats in two groups copulated with normal male rats. On days E17d, E18d and E19d, each rat of the intervention group received intragastric administration with active vitamin D (0.5 mg/kg), and they regained light and normal fodder. The morphology of fetal rat lungs on E20d and the 1-day-old neonatal rat lungs were observed by light microscope and electronic microscope. Results:Under the light microscope, smaller alveolar space, smaller respiratory membrane’s diameter and thicker alveolus mesenchyma were shown in lungs of vitamin deficient model group compared with control group(P < 0.05). Significant difference was found between the model and intervention group in neonatal lung (P < 0.05) but not in E20d fetal lung(P > 0.05). With the electronic microscope, fewer lamellar bodies but more glycogen deposition in intracytoplasm were detected in the fetal lung of E20d in experimental model group than controls. While in the model group of 1d after birth, despite of the number, the lamellar bodies were not as condensed as control, empty lamellar bodies were found. However, such changes were less sever after the supplement of active vitamin D. Conclution:Vitamin D deficiency in generation could inhibit the development of rat lung morphogenesis. Supplement with active vitamin D may reverse the inhibition.
查看全文   查看/发表评论  下载PDF阅读器
关闭