文章摘要
金曙蕾,苗登顺,韩群颖.Bmi-1基因敲除导致小鼠皮肤老化[J].南京医科大学学报,2009,29(3):312~316
Bmi-1基因敲除导致小鼠皮肤老化
Deletion of Bmi-1 gene leads to skin aging in mice
投稿时间:2008-08-09  
DOI:10.7655
中文关键词: Bmi-1  基因敲除  皮肤衰老
英文关键词: Bmi-1  gene knock-out  skin aging
基金项目:
作者单位
金曙蕾 南京医科大学人体解剖学系,江苏 南京 210029 
苗登顺  
韩群颖  
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中文摘要:
      目的:探讨Bmi-1在抗皮肤衰老中的作用?方法:使用组织学,免疫组织化学和Western-blot的实验方法比较分析了同窝2周和4周龄Bmi-1基因敲除纯合子小鼠和野生型小鼠皮肤表型的差异?结果:Bmi-1纯合子小鼠出现皮肤变薄?角质层增厚?皮下脂肪和胶原减少等衰老表型?通过增殖细胞核抗原(PCNA)免疫组织化学染色,发现Bmi-1纯合子小鼠中PCNA阳性细胞较同窝野生型小鼠明显减少?Western-blot结果显示细胞周期依赖性激酶抑制因子p16?p19和p27在Bmi-1纯合子小鼠皮肤组织中都明显升高?结论:Bmi-1能够通过抑制细胞周期依赖性激酶抑制因子的表达,刺激皮肤细胞增殖而发挥抗皮肤衰老的作用?
英文摘要:
      Objective:To investigate the role of Bmi-1 in preventing skin aging in mice. Methods:The phenotype of skin was compared between wild-type and Bmi-1 gene knock-out mice at 2 and 4 weeks of ages by histology, immunohistochemistry and Western-blot,respectively. Results:Bmi-1 gene knock-out mice displayed a premature aging phenotype in skin including thinner skin with hyperkeratosis of the epidermis, reduced collagen and subcutaneous fat deposition in skin matrix. Proliferating cell nuclear antigen immunopositive cells in skin were decreased significantly in Bmi-1 gene knock-out mice compared to their wild-type littermates. The expression of cell cycle dependent kinase inhibitive factors including p16,p19 and p27 was up-regulated markedly in skin from Bmi-1 gene knock-out mice. Conclusion:Bmi-1 plays an important role in preventing skin aging by inhibiting the expression of cell cycle dependent kinase inhibitive factors and stimulating cell proliferation.
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