文章摘要
徐 艳,陈群娥,王 军,王 伟,张文辉,周长美,朱红梅,张绍美.参附注射液对缺氧缺血性脑损伤新生大鼠白介素-18 和白介素-1β的影响[J].南京医科大学学报,2009,29(4):479~482
参附注射液对缺氧缺血性脑损伤新生大鼠白介素-18 和白介素-1β的影响
Effects of Shenfu Injection on the expression of Interleukin-18 and Interleukin-1β in neonatal rats with Hypoxic-ischemic brain damage
  
DOI:10.7655
中文关键词: 白细胞介素-18  白细胞介素-1β  缺氧缺血性脑损伤  新生大鼠  参附注射液
英文关键词: interleukin-18  interleukin-1β  hypoxic-ischemic brain damage  neonatal rats  Shenfu injection
基金项目:江苏省卫生厅科技计划重大项目基金资助(H200823)
作者单位
徐 艳 徐州医学院附属医院儿科,江苏 徐州 221002 
陈群娥  
王 军  
王 伟  
张文辉  
周长美  
朱红梅  
张绍美  
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中文摘要:
      目的:探讨参附注射液(SFI)对缺氧缺血性脑损伤(HIBD)新生大鼠白细胞介素-18(IL-18)和白细胞介素-1β(IL-1β)的影响?方法:参照Rice法制备新生大鼠HIBD 模型,将新生7日龄SD大鼠随机分为假手术组(S)?生理盐水对照组(C)?参附治疗组(SF),每组按术后观察时间点不同进一步分为3?12?24 h?6?14天 5个亚组,用ELISA方法检测病变侧脑组织中IL-18? IL-1β水平?结果:C组和SF组IL-1β(12?24 h?6天)?IL-18(24?6?14天)表达水平均较S组升高,其差别均有统计学意义(P < 0.01);IL-1β 在HI后12 h即达到高峰,随后开始下降,14天已降至正常水平?IL-18 在HI后24 h开始增加,6天达到高峰,之后开始下降,14天 仍高于S组(P < 0.01)?SF组IL-1β表达水平在12?24 h?6天较C组降低,其差别均有统计学意义(P < 0.01);SF组IL-18表达水平在24 h?6?14天较C组减少,其差别均有统计学意义(P < 0.01)?结论:HIBD后大鼠病变侧大脑皮层IL-18?IL-1β水平明显升高, 参附注射液(SFI)下调HIBD新生大鼠IL-18和 IL-1β的表达水平,SFI对新生大鼠HIBD有保护作用?
英文摘要:
      Objective:To investigate the effect of Shenfu injection(SFI) on the expression of Interleukin-18(IL-18) and Interleukin-1β(IL-1β) in neonatal rats with hypoxic - ischemic brain damage(HIBD), and clarify the protection of SFI for HI brain damage at the molecular level. Methods:Seven-day-old Sprague-Dawley rats were randomly assigned to sham operation group(S), normal sodium control group(C) and Shenfu injection treatment group(SF), and Models of postnatal 7-day Sprague Dawley(SD) rats with HIBD were established by the Rice method. They were further divided into five subgroups(n = 8 per subgroup) according to the time points 3 h,12 h,24 h,6d and 14 d. In each subgroup, rats were used to examine the level of IL-18,IL-1β in brain homogenates by ELISA. Results:The level of IL-1β was significantly higher in group C and group SF higher than that in group S at the time point 12 h, 24 h, 6 d(P < 0.01); The levels of IL-18 in group C and group SF were significantly higher than that of group S at the time point 24 h, 6 d, 14 d(P < 0.01); The expression of IL-1β in group C peaked at 12 h after HI insult and decreased after this, There was no difference in group S and group C at 14 d(P > 0.05); The expression of IL-18 in group C began to increase at 24 h after HI insult(P < 0.01), peaked at 6 d after HI insult and decreased after this. But IL-18 was still higher than group S(P < 0.01) at 14 d;the level of IL-1β in brain homogenates after HI insult in group SF were lower than group C at 12 h,24 h,6 d(P < 0.01); the level of IL-18 in brain homogenates after HI insult in group SF were lower than group C at every observed spot after 12 h(P < 0.01). Conclusion:The level of IL-18 and IL-1β in cerebral cortex had increased after HI insult in neonatal rats and SFI can decrease the level of IL-18 and IL-1β in cerebral cortex. SFI has a protedive function in neonatal rats after HI insult.
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